Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients

被引：0
作者：
Torbjörn Karlsson
机构：
[1] Uppsala University Hospital,Department of Haematology
来源：
Journal of Inflammation | / 14卷
关键词：
Hepcidin; Elisa; Anaemia; Iron deficiency; Inflammation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, a competitive hepcidin ELISA assay was evaluated for its ability to differentiate between iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients, using the absence of stainable bone marrow iron as the diagnostic criterion for iron deficiency. In addition, correlation coefficients for hepcidin versus C-reactive protein, ferritin and interleukin-6 were determined. The optimal cut-off for hepcidin was 21 μg/L, corresponding to sensitivity and specificity of 100% and 67%, respectively, for iron deficiency. For ferritin, a sensitivity and specificity of 91% and 83%, respectively, correspond to an optimal cut-off of 87 μg/L. Receiver operating characteristics curve analysis revealed that ELISA analysis of hepcidin is not superior to ferritin in the diagnosis of iron deficiency in elderly anaemic patients with concurrent inflammation. Hepcidin shows a strong positive correlation with ferritin, and also correlates positively with C-reactive protein in this patient population.
引用
收藏
相关论文
共 137 条
  • [21] Podust V(2016)The iron status of healthy south african population Eur J Clin Nutr 70 456-142
  • [22] Swinkels D(2004)The prevalence of anemia and iron deficiency is more common in breastfed infants than their mothers in Bhaktapur, Nepal Tohuko J Exp Med 202 135-1579
  • [23] Ganz T(2010)Soluble transferrin receptor and soluble transferrin receptor-ferritin index for evaluation of the iron status in elderly patients Clin Chem 56 1570-3680
  • [24] Olbina G(2011)Immunochemical and mass-spectrometry-based serum hepcidin assays for iron metabolism Arthritis Rheum 63 3672-1732
  • [25] Girelli D(2010)Hepcidin and hemoglobin content parameters in the diagnosis of iron deficiency in rheumatoid arthritis patients with anemia Clin Chem 56 1725-7
  • [26] Nemeth E(2015)A dual-monoclonal sandwich ELISA specific for hepcidin-25 Scand J Clin Lab Invest 75 1-42
  • [27] Westerman M(2011)Validation of a competitive ELISA assay for the quantification of human serum hepcidin Clin Exp Med 11 33-73
  • [28] Karlsson T(2014)Hepcidin expression in anemia of chronic disease and concomitant iron-deficiency anemia Eur J Haematol 94 67-552
  • [29] Guralnik J(2015)Hepcidin in tumor-related iron deficiency anemia and tumor-related anemia of chronic disease: pathogenic mechanisms and diagnosis Hematology 20 543-1276
  • [30] Eisenstaedt R(2004)Bone marrow iron distribution, hepcidin, and ferroportin expression in renal anemia J Clin Invest 113 1271-812
12345678910