Increased serum microRNAs are closely associated with the presence of microvascular complications in type 2 diabetes mellitus

被引:0
作者
Cheng Wang
Shujun Wan
Ting Yang
Dongmei Niu
Aisen Zhang
Cuihua Yang
Jialu Cai
Jia Wu
Jiaxi Song
Chen-Yu Zhang
Chunni Zhang
Junjun Wang
机构
[1] Jinling Hospital,Department of Clinical Laboratory
[2] Medical School of Nanjing University,Department of Geratology
[3] State Key Laboratory of Pharmaceutical Biotechnology,Department of Endocrinology
[4] Nanjing Advanced Institute for Life Sciences,undefined
[5] Nanjing University School of Life Sciences,undefined
[6] Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology,undefined
[7] Nanjing University,undefined
[8] the First Hospital Affiliated to Nanjing Medical University,undefined
[9] Jinling Hospital,undefined
[10] Medical School of Nanjing University,undefined
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Scientific Reports | / 6卷
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摘要
Circulating microRNAs (miRNAs) are emerging biomarkers for type 2 diabetes mellitus (T2DM). However, a comprehensive characterization of the serum miRNA profile in patients with T2DM-associated microvascular disease (T2DMC) has rarely been reported. In this study, we obtained serum samples from 184 T2DM patients (92 with microvascular complications and 92 free of complications) and 92 age/gender-matched controls. The levels of 754 miRNAs were initially analyzed using a TaqMan Low Density Array (TLDA) in three pooled samples from 24 T2DM patients, 24 T2DMC patients and 24 controls. Markedly upregulated miRNAs in the patients’ groups were subsequently validated individually by quantitative reverse-transcription PCR (RT-qPCR) in the same samples used for TLDA and further confirmed in another larger cohort consisting of 68 patients with T2DM, 68 patients with T2DMC and 68 controls. Five miRNAs were significantly upregulated in T2DM patients (p < 0.05) including miR-661, miR-571, miR-770-5p, miR-892b and miR-1303. Moreover, the levels of the five miRNAs were higher in patients with complications than in those without complications. Regression analyses revealed the five miRNAs were significantly correlated with microvascular complications (p < 0.05). The five serum miRNAs identified in our study hold potential as auxiliary biomarkers and novel risk factors for T2DM-associated microvascular complications.
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  • [1] Ashcroft FM(2012)Diabetes mellitus and the beta cell: the last ten years Cell 148 1160-1171
  • [2] Rorsman P(2004)Global prevalence of diabetes: estimates for the year 2000 and projections for 2030 Diabetes care 27 1047-1053
  • [3] Wild S(2014)Global estimates of diabetes prevalence for 2013 and projections for 2035 Diabetes research and clinical practice 103 137-149
  • [4] Roglic G(2006)Oxidative stress in type 2 diabetes: the role of fasting and postprandial glycaemia International journal of clinical practice 60 308-314
  • [5] Green A(2010)Organic nitrates and nitrate resistance in diabetes: the role of vascular dysfunction and oxidative stress with emphasis on antioxidant properties of pentaerithrityl tetranitrate Experimental diabetes research 2010 213176-94
  • [6] Sicree R(2013)MicroRNAs: potential mediators and biomarkers of diabetic complications Free radical biology & medicine 64 85-256
  • [7] King H(2013)Elevated serum beta2-glycoprotein-I-lipoprotein(a) complexes levels are associated with the presence and complications in type 2 diabetes mellitus Diabetes research and clinical practice 100 250-842
  • [8] Guariguata L(2008)Biological principles of microRNA-mediated regulation: shared themes amid diversity Nature reviews. Genetics 9 831-701
  • [9] Wright E(2008)Role of microRNAs in diabetes Biochimica et biophysica acta 1779 697-1837
  • [10] Scism-Bacon JL(2011)Diabetes complications: the microRNA perspective Diabetes 60 1832-1006