Emergence and genetic analysis of variant pathogenic 4/91 (serotype 793/B) infectious bronchitis virus in Egypt during 2019

被引:0
作者
Mohammed A. Rohaim
Rania F. El Naggar
Mohamed M. Hamoud
Abdel-Hamid I. Bazid
Abdulrhman M. Gamal
Samah E. Laban
Mohammed A. Abdel-Sabour
Shimaa A. E. Nasr
Manal M. Zaki
Muhammad Z. Shabbir
Osama K. Zahran
Muhammad Munir
机构
[1] Cairo University,Department of Virology, Faculty of Veterinary Medicine
[2] Lancaster University,Division of Biomedical and Life Sciences, Faculty of Health and Medicine
[3] University of Sadat City,Department of Virology, Faculty of Veterinary Medicine
[4] Cairo University,Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine
[5] Cairo University,Department of Animal Hygiene and Veterinary Management, Faculty of Veterinary Medicine
[6] Veterinary Serum and Vaccine Research Institute,Quality Operations Laboratory
[7] University of Veterinary and Animal Sciences,undefined
来源
Virus Genes | 2019年 / 55卷
关键词
Infectious bronchitis virus; Curling and dwarfism; Identity; Recombination; Emergence; Monitoring;
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中图分类号
学科分类号
摘要
Infectious bronchitis virus (IBV) affects both vaccinated and unvaccinated flocks worldwide, with a significant impact on the poultry industry. The aim of the present study is to characterize an emerging variant pathogenic IBV originating from field outbreaks in vaccinated Egyptian layer flock. Samples were collected from disease-suspected flock with a history of administration of live and inactivated IBV vaccines (Ma5 type). Virus propagation in embryonated chicken eggs (ECEs), after three successive passages, revealed typical IBV lesions such as curling and dwarfism. The reported isolate was identified by a real-time reverse transcriptase PCR assay targeting nucleocapsid (N) gene and, further characterized by full-length spike (S1) gene sequencing. Phylogenetic analysis revealed clustering of the isolated virus within 4/91 genotype of GI-13 lineage. Deduced amino acid sequences identity revealed 75–76% and 88–90% similarity with the currently used classic (H120, Ma5, and M41) and variant vaccine strains (4/91 and CR88) in Egypt, respectively. Recombination analysis gave an evidence for distinct patterns of origin for the studied isolate providing another example of intra-genotypic recombination among IBVs and the first example of recombination within the GI-13 lineage in the Egyptian field. The studied isolate (IBV/CK/EG/Fadllah-10/2019) emerged as a result of recombination between the variant group (Egy/var I genotype, GI-23 lineage) as a major parent and the CR88 variant vaccine strain (4/91 genotype, GI-13 lineage) as minor parent. Our data suggest that both mutation and recombination may be contributing to the emergence of IBV variants which ascertain the importance of disease monitoring in vaccinated flocks as well as re-appropriation for the current vaccine strategies.
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页码:720 / 725
页数:5
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