Ultrasonically induced cell damage and membrane lipid peroxidation by photofrin II: Mechanism of sonodynamic activation

被引：17
作者：
Yumita N. [1 ]
Umemura S.-I. [2 ]
机构：
[1] School of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510
[2] Central Research Laboratory, Hitachi, Kokubunji, Tokyo 185-8601
来源：
Journal of Medical Ultrasonics | 2004年 / 31卷 / 1期
关键词：
Active oxygen; Cell damage; Lipid peroxidation; Photofrin II; Ultrasound;
D O I：
10.1007/s10396-003-0003-6
中图分类号：
学科分类号：
摘要：
Purpose. Ultrasonically induced cell damage and active oxygen generation with photofrin II were compared using the same in vitro insonation setup. Methods. Sarcoma 180 cells suspended in an air-saturated phosphate-buffered saline solution were exposed to ultrasound at 2MHz for not more than 60s in the presence and absence of photofrin II. The trypan blue exclusion test was used to determine viability. Lipid peroxidation in cell membranes was estimated by measuring the quantity of reactive substance produced from thiobarbituric acid added immediately after the cells had been exposured to ultrasound. Results. Significant enhancement of the rates of ultrasonically induced cell damage and lipid peroxidation was demonstrated with photofrin II (20-80 μg/ml), and the two rates were closely associated. Enhancement of both rates by photofrin II was suppressed by 10mM of histidine. Conclusion. These results suggest that ultrasonically generated active oxygen is a primary factor in ultrasonically induced cell damage in the presence of photofrin II. © The Japan Society of Ultrasonics in Medicine 2004.
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页码：35 / 40
页数：5
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  • [1] Yumita N., Nishigaki R., Umemura K., Et al., Hematoporphyrin as a sensitizer of cell damaging effect of ultrasound, Jpn J Cancer Res, 80, pp. 219-222, (1989)
  • [2] Yumita N., Nishigaki R., Umemura K., Et al., Synergetic effect of ultrasound and hematoporphyrin on sarcoma 180, Jpn J Cancer Res, 81, pp. 304-308, (1990)
  • [3] Yumita N., Umemura S., Nishigaki R., Ultrasonically induced cell damage enhanced by photofrin II: Mechanism of sonodynamic activation, In Vivo, 14, pp. 425-429, (2000)
  • [4] Yumita N., Nishigaki R., Umemura S., Sonodynamically induced antitumor effect of photofrin II on colon 26 carcinoma, Cancer Res Clin Oncol, 126, pp. 601-606, (2000)
  • [5] Tachibana K., Uchida T., Hisano S., Et al., Eliminating adult T-cell leukaemia cells with ultrasound, Lancet, 349, (1997)
  • [6] Dougherty T.J., Photodynamic therapy, Photochem Photobiol, 58, pp. 895-905, (1993)
  • [7] Bellnier D.A., Ho Y.K., Panday R.K., Et al., Distribution and elimination of photofrin II in mice, Photochem Photobiol, 50, pp. 221-228, (1989)
  • [8] Yumita N., Nishigaki R., Umemura K., Et al., Sonochemical activation of hematoporphyrin: An ESR study, Radiat Res, 138, pp. 171-176, (1994)
  • [9] Umemura S., Yumita N., Nishigaki R., Enhancement of ultrasonically induced cell damage by a gallium-porphyrin complex, ATX70, Jpn J Cancer Res, 84, pp. 582-588, (1993)
  • [10] Yumita N., Umemura S., Magario N., Et al., Membrane lipid peroxidation as a mechanism of sonodynamically induced erythrocyte lysis, Int J Radiat Biol, 69, pp. 397-404, (1996)
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