Molecular genetic analysis of quinolone resistance-determining region of DNA Gyrase-A in fluoroquinolones resistant Klebsiella pneumoniae based on GenBank data and reported studies

Infectious diseases caused by fluoroquinolone-resistant strains of Klebsiella pneumoniae is a considerable challenge in healthcare issues. Mutations in gyrA gene nucleotide sequences are the most important subject in the field of bacterial antibiotic-resistance mechanisms. In this study 200 cases of Klebsiella pneumoniae have been studied. For this purpose, 80 partial sequences of gyrA belonging to fluoroquinolone-resistant strains of Klebsiella pneumoniae obtained from the National Center for Biotechnology Information (NCBI) were analyzed via bioinformatic software and 120 ready cases from several reported studies directly imported into this investigation. Results showed that, two codons of Serine-83 and Aspartic acid-87 have the most frequent mutations including Ser-83 → Phe (33%), Ser-83 → Tyr (20%) and Asp-87 → Asn (25%), Asp-87 → Ala (8%) that have high hydrophobicity in comparison with normal amino acids. According to the results of this investigation, the high distribution of hot spot mutations in gyrA gene sequences may lead to critical problems in the field of antibiotic therapy. Thus, the use of bioinformatics and statistical analyses are prompt approaches to perceive and control the probable drug-resistant bacterial strains.