Efficacy of nanoparticles against purple blotch (Alternaria porri) of onion

被引:2
作者
Chaithanya G. [1 ]
Kumar A. [1 ]
Vijay D. [1 ]
Singh P.K. [3 ]
Hussain Z. [2 ]
Basu S. [1 ]
Lal S.K. [1 ]
机构
[1] Division of Seed Science and Technology, ICAR-Indian Agricultural Research Institute, New Delhi
[2] Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi
[3] Centre for Protected Cultivation Technology, ICAR-Indian Agricultural Research Institute, New Delhi
关键词
Nano-priming; Nanoparticles; Onion seed; Purple blotch; Zinc oxide;
D O I
10.1007/s42360-023-00632-x
中图分类号
学科分类号
摘要
Onion (Allium cepa), belonging to family, Amaryllidaceae is an essential crop in everyday life. However, its seeds are considered to be short lived due to its thin, shrunken and damaged seed coat, which makes it susceptible to fungal infection. An effort was made to enhance the seed quality of onion through management of seed borne disease, Purple Blotch of onion caused by Alternaria porri, though a novel method called Nano-priming. Experimentations were done to assess the antifungal activity of Zinc Oxide (ZnO) and Graphene Oxide (GO) nanoparticles (NPs) at different concentrations i.e., ZnO (25, 50, 100, 250 and 500 ppm) and GO (50, 100, 250, 500 and 1000 ppm) via mycelial reduction method and Agar well diffusion method. A ZnO @250 ppm significantly inhibited the in vitro growth of pathogen, as compared to Graphene oxide and the two positive controls-SAAF@ 0.1% a.i. (Carbendazim 12% + Mancozeb 63%) and Carbendazim (0.1% a.i.). Further, the seeds were artificially inoculated with spores of A. porri, followed by nano-priming of ZnO at different concentrations and evaluated for frequency of seed infection after 7 days. The results unveiled that ZnO NPs at concentration of 250 ppm and above inhibited the growth of test pathogen. Hence, the ZnO nano-priming at 250 ppm was found to be effective in enhancing the seed quality as well as protection against the Purple Blotch disease in onion. © 2023, Indian Phytopathological Society.
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页码:845 / 852
页数:7
相关论文
共 29 条
  • [1] Alam S.N., Sharma N., Kumar L., Synthesis of graphene oxide (GO) by modified hummers method and its thermal reduction to obtain reduced graphene oxide (rGO), Graphene, 6, 1, pp. 1-18, (2017)
  • [2] Alvarez-Peral F.J., Zaragoza O., Pedreno Y., Arguelles J.C., Protective role of trehalose during severe oxidative stress caused by hydrogen peroxide and the adaptive oxidative stress response in Candida albicans, Microbiology, 148, 8, pp. 2599-2606, (2002)
  • [3] Alwan R.M., Kadhim Q.A., Sahan K.M., Ali R.A., Mahdi R.J., Kassim N.A., Jassim A.N., Synthesis of zinc oxide nanoparticles via sol–gel route and their characterization, Nanosci Nanotechnol, 5, 1, pp. 1-6, (2015)
  • [4] Azimi S., Behin J., Abiri R., Rajabi L., Derakhshan A.A., Karimnezhad H., Synthesis, characterization and antibacterial activity of chlorophyllin functionalized graphene oxide nanostructures, Sci Adv Mater, 6, 4, pp. 771-781, (2014)
  • [5] Boswell V.R., Toole E.H., Toole V.K., Fisher D.F., A study of rapid deterioration of vegetable seeds and methods for its prevention, Technical Bull, 708, (1940)
  • [6] Chen J., Sun L., Cheng Y., Lu Z., Shao K., Li T., Han H., Graphene oxide-silver nanocomposite: novel agricultural antifungal agent against Fusarium graminearum for crop disease prevention, ACS Appl Mater Interfaces, 8, 36, pp. 24057-24070, (2016)
  • [7] Elemike E.E., Uzoh I.M., Onwudiwe D.C., Babalola O.O., The role of nanotechnology in the fortification of plant nutrients and improvement of crop production, Appl Sci, 9, 3, (2019)
  • [8] Ellis M.B., Holliday P., Alternaria porri [Descriptions of Fungi and Bacteria], Descriptions of Fungi and Bacteria, 1, 25, (1970)
  • [9] Gupta R.B.L., Pathak V.N., Yield losses in onions due to purple blotch disease caused by Alternaria porri, Phytophylactica, 20, 1, pp. 21-23, (1988)
  • [10] Hafeez A., Razzaq A., Mahmood T., Jahanzeb H.M., Potential of copper nanoparticles to increase growth and yield of wheat, J Nanosci Adv Technol, 1, 1, pp. 6-11, (2015)