Colchicine and oryzalin mediated chromosome doubling in different genotypes of Miscanthus sinensis

被引：82

作者：

Karen Koefoed Petersen

Per Hagberg

Kell Kristiansen

机构：

[1] Department of Horticulture, Research Centre Aarslev, Danish Inst. of Agric. Sciences, DK-5792 Aarslev

[2] Nilsson-Ehle Laboratory, Svalöf Weibull AB

来源：

Plant Cell, Tissue and Organ Culture | 2003年 / 73卷 / 2期

关键词：

Antimitotic agent; Callus formation; Chimera; Explant type; Flow cytometry; Plant regeneration; Polyploid;

D O I：

10.1023/A:1022854303371

中图分类号：

学科分类号：

摘要：

Different explant materials were treated with antimitotic agents to induce chromosome doubling in several Miscanthus sinensis clones. In vitro propagated plants established in soil, in vitro shoots, embryogenic callus, shoot apices and leaf explants were treated with different concentrations of colchicine or oryzalin. No tetraploids were obtained after antimitotic treatment of plants established in soil. The percentage of chromosome doubled plants after antimitotic treatment of single in vitro shoots was genotype dependent. Rooted in vitro plantlets were not a suitable target for antimitotic treatment, due to a high frequency of ploidy chimeras. Many tetraploid plants were regenerated after antimitotic treatment at the callus and explant level, but the efficiency was genotype dependent, primarily due to differences in the ability to form regenerable callus and to regenerate plants from embryogenic callus. Treatment of shoot apices with colchicine was the most efficient and reproducible system in the four genotypes tested. It was possible to repeatedly use the same colchicine-containing medium without any reduction in the induction of regenerable callus or in the percentage of tetraploids, thereby minimising the handling of this very toxic compound.

引用

页码：137 / 146

页数：9

共 21 条

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