A ribonuclease from the wild mushroom Boletus griseus

被引：0
作者：
Hexiang Wang
T. B. Ng
机构：
[1] China Agricultural University,State Key Laboratory of Agrobiotechnology, Department of Microbiology, College of Biological Science
[2] The Chinese University of Hong Kong,Department of Biochemistry, Faculty of Medicine
[3] Shatin,undefined
来源：
Applied Microbiology and Biotechnology | 2006年 / 72卷
关键词：
Fruiting Body; Pleurotus; RNase Activity; Pleurotus Ostreatus; Wild Mushroom;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A ribonuclease (RNase) with a molecular mass of 29 kDa and cospecific for poly A and poly U was isolated from fruiting bodies of the mushroom Boletus griseus. Its N-terminal sequence exhibited some similarity to those of RNases from the mushrooms Irpex lacteus and Lentinus edodes. The RNase was adsorbed on diethylaminoethyl-cellulose, Q-Sepharose, and Affi-gel blue gel and was unadsorbed on CM-cellulose. The enzyme exhibited a temperature optimum between 60 and 70°C and a pH optimum at 3.5.
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页码：912 / 916
页数：4
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