Is mouse embryonic stem cell technology obsolete?

被引：0
作者：
William C Skarnes
机构：
[1] Wellcome Trust Genome Campus,Stem Cell Engineering, Wellcome Trust Sanger Institute
来源：
Genome Biology | / 16卷
关键词：
Embryonic Stem Cell; Homologous Recombination; Mouse Embryonic Stem Cell; Cluster Regularly Interspaced Short Palindromic Repeat; Donor Plasmid;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Injection of recombinant Cas9 protein and synthetic guide RNAs into mouse zygotes has been shown to facilitate gene disruption and knock-ins using the CRISPR system. These technologies may soon displace genetic modification using embryonic stem cells.
引用
收藏
相关论文
共 50 条
  • [11] Integrins regulate mouse embryonic stem cell self-renewal
    Hayashi, Yohei
    Furue, Miho Kusuda
    Okamoto, Tetsuji
    Ohnuma, Kiyoshi
    Myoishi, Yasufumi
    Fukuhara, Yasuaki
    Abe, Takanori
    Sato, J. Denry
    Hata, Ryu-Ichiro
    Asashima, Makoto
    STEM CELLS, 2007, 25 (12) : 3005 - 3015
  • [12] PiggyBac Mediated Multiplex Gene Transfer in Mouse Embryonic Stem Cell
    Lu, Xibin
    Huang, Wei
    PLOS ONE, 2014, 9 (12):
  • [13] Mitochondria structural reorganization during mouse embryonic stem cell derivation
    Lyubov A. Suldina
    Ksenia N. Morozova
    Aleksei G. Menzorov
    Elena A. Kizilova
    Elena Kiseleva
    Protoplasma, 2018, 255 : 1373 - 1386
  • [14] Selection and dynamics of embryonic stem cell integration into early mouse embryos
    Alexandrova, Stoyana
    Kalkan, Tuzer
    Humphreys, Peter
    Riddell, Andrew
    Scognamiglio, Roberta
    Trumpp, Andreas
    Nichols, Jennifer
    DEVELOPMENT, 2016, 143 (01): : 24 - 34
  • [15] Differentiation of mast cell subpopulations from mouse embryonic stem cells
    Westerberg, Christine Moller
    Ulleras, Erik
    Nilsson, Gunnar
    JOURNAL OF IMMUNOLOGICAL METHODS, 2012, 382 (1-2) : 160 - 166
  • [16] Electron microscopic study of mouse embryonic stem cell-derived cardiomyocytes
    Taha, Masoumeh Fakhr
    Valojerdi, Mojtaba Rezazadeh
    Hatami, Leili
    Javeri, Arash
    CYTOTECHNOLOGY, 2012, 64 (02) : 197 - 202
  • [17] Nitric oxide repression of Nanog promotes mouse embryonic stem cell differentiation
    Mora-Castilla, S.
    Tejedo, J. R.
    Hmadcha, A.
    Cahuana, G. M.
    Martin, F.
    Soria, B.
    Bedoya, F. J.
    CELL DEATH AND DIFFERENTIATION, 2010, 17 (06) : 1025 - 1033
  • [18] Identification of differentially expressed genes in mouse embryonic stem cell under hypoxia
    Hwang, Su Jung
    Lee, Hyo-Jong
    GENES & GENOMICS, 2021, 43 (04) : 313 - 321
  • [19] Identification of differentially expressed genes in mouse embryonic stem cell under hypoxia
    Su Jung Hwang
    Hyo-Jong Lee
    Genes & Genomics, 2021, 43 : 313 - 321
  • [20] Nitric oxide repression of Nanog promotes mouse embryonic stem cell differentiation
    S Mora-Castilla
    J R Tejedo
    A Hmadcha
    G M Cahuana
    F Martín
    B Soria
    F J Bedoya
    Cell Death & Differentiation, 2010, 17 : 1025 - 1033
12345