Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells

被引：0
作者：
Feng Zhang
Yonghua Cui
Pingping Cao
机构：
[1] Tongji Hospital,Department of Otorhinolaryngology
[2] Tongji Medical College,Head and Neck Surgery
[3] Huazhong University of Science and Technology,undefined
来源：
Journal of Huazhong University of Science and Technology [Medical Sciences] | 2008年 / 28卷
关键词：
quercetin; HEN1 cells; apoptosis; cell cycle; caspase-3;
D O I：
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中图分类号：
学科分类号：
摘要：
The effect of quercetin (Que) on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells was investigated. Inhibition rate of quercetin on HEN1 was assayed by MTT method, apoptosis by flow cytometry (FCM), and the caspase-3 expression of each group by colorimetry set respectively. Quercetin inhibited HEN1 cells in in a dose-(r=0.709, P<0.01) and time-dependent manner (r=0.703, P<0.01). The ratio of apoptotic and necrosis cells was increased in the cells treated with quercetin. Cell cycle was specificly arrested in G2/M phase. Apoptosis cusp was revealed by FCM. The activity of caspase-3 was significantly up-regulated in 5 groups treated with quecetin as compared with control group (P<0.05). It was concluded that the growth inhibition of quercetin was highly related to cell cycle arrest at the G2/M phase and induction of caspase-dependent apoptosis in human nasopharyngeal carcinoma HEN1 cells.
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页码：369 / 372
页数：3
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