Genomic DNA Fingerprinting of Oenococcus oeni Strains by Pulsed-Field Gel Electrophoresis and Randomly Amplified Polymorphic DNA-PCR

被引：0

作者：

Giacomo Zapparoli

Cristina Reguant

Albert Bordons

Sandra Torriani

Franco Dellaglio

机构：

[1] Dipartimento Scientifico e Tecnologico,

[2] Università degli Studi di Verona,undefined

[3] strada Le Grazie,undefined

[4] 37134 Verona,undefined

[5] Italy ,undefined

[6] Departament de Bioquímica i Biotecnologia,undefined

[7] Universitat Rovira i Virgili,undefined

[8] Pl. Imperial Tarraco,undefined

[9] 1,undefined

[10] 43005 Tarragona,undefined

[11] Spain ,undefined

[12] Istituto di Industrie Agrarie,undefined

[13] Università degli Studi di Catania,undefined

[14] via Santa Sofia 98,undefined

[15] 95124 Catania,undefined

[16] Italy ,undefined

来源：

Current Microbiology | 2000年 / 40卷

关键词：

Genetic Diversity; Electrophoresis; Geographical Origin; Oligonucleotide Primer; Suitable Method;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Genetic diversity of 60 Oenococcus oeni strains from different wines was evaluated by numerical analysis of (i) pulsed-field gel electrophoresis (PFGE) patterns with endonuclease ApaI and (ii) randomly amplified polymorphic DNA (RAPD)-PCR fingerprints with four oligonucleotide primers. Sixty-two percent of the strains could be distinguished by PFGE, whereas most strains were identified by distinct RAPD-PCR profiles and associated according to the geographical origin. Because of its rapidity and reliability, RAPD-PCR appeared to be a suitable method for typing and monitoring O. oeni strains in winemaking.

引用

页码：351 / 355

页数：4