A spectrophotometric method for quantitative determination of lactulose in pharmaceutical preparations

被引：2
作者：
Mir Azam Khan
Zafar Iqbal
Mohammad Rasul Jan
Jasmin Shah
Waqar Ahmad
Zia Ul Haq
机构：
[1] University of Peshawar,Department of Chemistry
[2] University of Peshawar,Department of Pharmacy
[3] Malakand University,Department of Pharmacy
[4] Libra Pvt Ltd. Industrial Estate,undefined
[5] Drug Control Administration,undefined
[6] Ministry of Health,undefined
来源：
Journal of Analytical Chemistry | 2006年 / 61卷
关键词：
Hydrolysis; Analytical Chemistry; Absorption Peak; Commercial Product; Acidic Condition;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A simple spectrophotometric assay for the quantification of lactulose in pharmaceutical preparations was developed. The method is based on hydrolysis of lactulose under acidic conditions. The hydrolyzed product reacts with resorcinol, giving absorption peaks at 398 and 480 nm. Both absorption wavelengths can be used for the determination of lactulose. The limit of detection of lactulose at 398 nm and 480 nm was 0.075 μg mL−1 and 0.65 μg mL−1, respectively. The calibration was linear in the range of 5–25 μg mL−1. Analytical conditions were optimized, and the method was validated for analysis of pharmaceutical preparations. The determined amount of lactulose was found to be in good agreement with labeled claims in commercial products. The proposed method is economical, convenient, and suitable for the quantification of lactulose in pharmaceutical preparations.
引用
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页码：32 / 36
页数：4
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