A spectrophotometric method for quantitative determination of lactulose in pharmaceutical preparations

被引:2
作者
Mir Azam Khan
Zafar Iqbal
Mohammad Rasul Jan
Jasmin Shah
Waqar Ahmad
Zia Ul Haq
机构
[1] University of Peshawar,Department of Chemistry
[2] University of Peshawar,Department of Pharmacy
[3] Malakand University,Department of Pharmacy
[4] Libra Pvt Ltd. Industrial Estate,undefined
[5] Drug Control Administration,undefined
[6] Ministry of Health,undefined
来源
Journal of Analytical Chemistry | 2006年 / 61卷
关键词
Hydrolysis; Analytical Chemistry; Absorption Peak; Commercial Product; Acidic Condition;
D O I
暂无
中图分类号
学科分类号
摘要
A simple spectrophotometric assay for the quantification of lactulose in pharmaceutical preparations was developed. The method is based on hydrolysis of lactulose under acidic conditions. The hydrolyzed product reacts with resorcinol, giving absorption peaks at 398 and 480 nm. Both absorption wavelengths can be used for the determination of lactulose. The limit of detection of lactulose at 398 nm and 480 nm was 0.075 μg mL−1 and 0.65 μg mL−1, respectively. The calibration was linear in the range of 5–25 μg mL−1. Analytical conditions were optimized, and the method was validated for analysis of pharmaceutical preparations. The determined amount of lactulose was found to be in good agreement with labeled claims in commercial products. The proposed method is economical, convenient, and suitable for the quantification of lactulose in pharmaceutical preparations.
引用
收藏
页码:32 / 36
页数:4
相关论文
共 50 条
[21]   Galvanostatic coulometric determination of aromatic amine derivatives in pharmaceutical preparations using electrochemically generated bromine [J].
Abdullin, IF ;
Chernysheva, NN ;
Budnikov, GK .
JOURNAL OF ANALYTICAL CHEMISTRY, 2002, 57 (07) :629-631
[22]   Galvanostatic Coulometric Determination of Aromatic Amine Derivatives in Pharmaceutical Preparations Using Electrochemically Generated Bromine [J].
I. F. Abdullin ;
N. N. Chernysheva ;
G. K. Budnikov .
Journal of Analytical Chemistry, 2002, 57 :629-631
[23]   A novel kinetic cholinesterase-inhibition based method for quantification of biperiden in pharmaceutical preparations [J].
Djuric, Vladan R. ;
Mitic, Violeta D. ;
Deletic, Nebojsa R. ;
Ilic, Marija D. ;
Cirkovic, Bratislav M. ;
Stankov-Jovanovic, Vesna P. .
HEMIJSKA INDUSTRIJA, 2020, 74 (04) :265-271
[24]   Investigation of new indirect spectrophotometric method for the determination of carbofuran in carbamate pesticides [J].
Jan, MR ;
Shah, J ;
Khan, H .
CHEMOSPHERE, 2003, 52 (09) :1623-1626
[25]   Spectrophotometric determination of pyrazidole [J].
Illarionova, EA ;
Syrovatskii, IP ;
Artasyuk, EM .
JOURNAL OF ANALYTICAL CHEMISTRY, 2004, 59 (06) :557-559
[26]   Spectrophotometric Determination of Pyrazidole [J].
E. A. Illarionova ;
I. P. Syrovatskii ;
E. M. Artasyuk .
Journal of Analytical Chemistry, 2004, 59 :557-559
[27]   A versatile, quantitative analytical method for pharmaceutical relevant lipids in drug delivery systems [J].
Jeschek, Dominik ;
Lhota, Gabriele ;
Wallner, Jakob ;
Vorauer-Uhl, Karola .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2016, 119 :37-44
[28]   An Overview of Green Chromatographic Analysis from Pharmaceutical Preparations [J].
Dogan, Aysegul ;
Eylem, Cemil Can ;
Akduman, Nursabah E. Basci .
CURRENT ANALYTICAL CHEMISTRY, 2021, 17 (09) :1260-1277
[29]   Determination of vitamins E and D3 in some preparations by high-performance liquid chromatography with indirect spectrophotometric detection [J].
Lazareva, EE ;
Brykina, GD ;
Shpigun, OA .
JOURNAL OF ANALYTICAL CHEMISTRY, 2002, 57 (07) :616-619
[30]   Determination of Vitamins E and D3 in Some Preparations by High-Performance Liquid Chromatography with Indirect Spectrophotometric Detection [J].
E. E. Lazareva ;
G. D. Brykina ;
O. A. Shpigun .
Journal of Analytical Chemistry, 2002, 57 :616-619