Establishment and characterization of a human liposarcoma cell line (HTLS) from the retroperitoneal liposarcoma.

被引：1

作者：

Kiguchi K. ^{[1
]}

Ishiwata I. ^{[1
]}

Ishiwata E. ^{[1
]}

Koshitaka Y. ^{[1
]}

Ohara T. ^{[1
]}

Okuda Y. ^{[1
]}

Sato S. ^{[1
]}

Kobayashi Y. ^{[1
]}

Ishizuka B. ^{[1
]}

Ishikawa H. ^{[1
]}

机构：

[1] St. Marianna University,Department of Obstetrics & Gynecology

[2] Ishiwata Obstetrics & Gynecology Hospital,Department of Obstetrics and Gynecology

[3] Fujita Health University School of Medicine,Department of Pathology

[4] Machida Municipal Hospital,Department of Anatomy

[5] Jikei University,undefined

[6] School of Medicine,undefined

来源：

Human Cell | 2005年 / 18卷 / 1期

关键词：

retroperitoneal liposarcoma; cell line; biological characteristics;

D O I：

10.1111/j.1749-0774.2005.tb00056.x

中图分类号：

学科分类号：

摘要：

A cell line designated HTLS was established from the retroperitoneal liposarcoma. The HTLS line showed stable proliferation without interruption for 2 years and subcultivated over 35 times. The cells were elongated fibrous and spindle in shape, and neoplastic and pleomorphic features. The multinucleated giant cells with fine cytoplasm were seen. The cells proliferated slowly and the population doubling time was about 90 hours. The chromosome number showed a wide distribution of aneuploidy, the mode was hyperdiploid range (51-52), and many marker chromosomes were observed. The cells were transplantable into the submucosa of immunesuppressed hamster's cheek pouch and produced liposarcoma, while were not transplantable into subcutis of nude mice

引用

页码：45 / 52

页数：7

共 36 条

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