Expression profiling of MicroRNAs in hippocampus of rats following traumatic brain injury

被引：0
作者：
Ting-yi Sun
Xiao-rui Chen
Zi-long Liu
Li-li Zhao
Yong-xiang Jiang
Guo-qiang Qu
Rong-shuai Wang
Si-zhe Huang
Liang Liu
机构：
[1] Huazhong University of Science and Technology,Department of Forensic Medicine, Tongji Medical College
[2] Forensic Expertise Centre of Xiling District Public Security Bureau,undefined
来源：
Journal of Huazhong University of Science and Technology [Medical Sciences] | 2014年 / 34卷
关键词：
forensic clinical medicine; traumatic brain injury; microRNA; microarray technique; hippocampus;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The changes of microRNA expression in rat hippocampus after traumatic brain injury (TBI) were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI: 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regulated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.
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页码：548 / 553
页数：5
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