Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells

被引：0
作者：
Hui-Fang Chang
Hung-Tien Cheng
Huai-Yi Chen
Wing Kiu Yeung
Ji-Yen Cheng
机构：
[1] Academia Sinica,Research Center for Applied Sciences
[2] National Tsing Hua University,Department of Engineering and System Science
[3] Taiwan International Graduate Program,Nano Science and Technology Program
[4] Academia Sinica and National Tsing Hua University,Department of Mechanical and Mechatronic Engineering
[5] National Taiwan Ocean University,Institute of Biophotonics
[6] National Yang-Ming University,College of Engineering
[7] Chang Gung Engineering,undefined
来源：
Scientific Reports | / 9卷
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摘要：
Adenocarcinoma, large cell carcinoma and squamous cell carcinoma are the most commonly diagnosed subtypes of non-small cell lung cancers (NSCLC). Numerous lung cancer cell types have exhibited electrotaxis under direct current electric fields (dcEF). Physiological electric fields (EF) play key roles in cancer cell migration. In this study, we investigated electrotaxis of NSCLC cells, including human large cell lung carcinoma NCI-H460 and human lung squamous cell carcinoma NCI-H520 cells. Non-cancerous MRC-5 lung fibroblasts were included as a control. After dcEF stimulation, NCI-H460 and NCI-H520 cells, which both exhibit epithelial-like morphology, migrated towards the cathode, while MRC-5 cells, which have fibroblast-like morphology, migrated towards the anode. The effect of doxycycline, a common antibiotic, on electrotaxis of MRC-5, NCI-H460 and NCI-H520 cells was examined. Doxycycline enhanced the tested cells’ motility but inhibited electrotaxis in the NSCLC cells without inhibiting non-cancerous MRC-5 cells. Based on our finding, further in-vivo studies could be devised to investigate the metastasis inhibition effect of doxycycline in an organism level.
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[1]  
Wong MCS(2017)Incidence and mortality of lung cancer: global trends and association with socioeconomic status Scientific reports. 7 584-594
[2]  
Lao XQ(2008)Non-small cell lung cancer: epidemiology, risk factors, treatment, and survivorship Mayo Clinic proceedings. 83 1059-1068
[3]  
Ho KF(2001)The new World Health Organization classification of lung tumours The European respiratory journal. 18 902-907
[4]  
Goggins WB(2015)Variation in causes of death in patients with non-small cell lung cancer according to stage and time since diagnosis Annals of oncology: official journal of the European Society for Medical Oncology. 26 402-418
[5]  
Tse SLA(2015)Cancer metastases: challenges and opportunities Acta pharmaceutica Sinica. B. 5 23-34
[6]  
Molina JR(2011)Initial steps of metastasis: cell invasion and endothelial transmigration Mutation research. 728 1068-1075
[7]  
Yang P(2000)Electrophysiological recordings from the rat prostate gland BJU international. 86 156-167
[8]  
Cassivi SD(2003): identified single-cell and transepithelial (lumen) potentials The Prostate. 54 3510-3516
[9]  
Schild SE(2009)Ionic and pharmacologic characteristics of epithelial cells in a semi-intact preparation of the rat ventral prostate gland Biosensors & bioelectronics. 24 817-830
[10]  
Adjei AA(2014)Electrotaxis of lung cancer cells in a multiple-electric-field chip Integrative biology: quantitative biosciences from nano to macro. 6 4942-4946
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