1H, 13C, and 15N resonance assignments of FAS1-IV domain of human periostin, a component of extracellular matrix proteins

被引：0
作者：
Hyosuk Yun
Eun-Hee Kim
Chul Won Lee
机构：
[1] Chonnam National University,Department of Chemistry
[2] Protein Structure Group,undefined
[3] Korea Basic Science Institute,undefined
来源：
Biomolecular NMR Assignments | 2018年 / 12卷
关键词：
NMR resonance assignment; Periostin; Extracellular matrix; FAS1 domain;
D O I：
暂无
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学科分类号：
摘要：
Periostin, an extracellular matrix protein, is secreted by fibroblasts and is overexpressed in various types of cancers. The four internal repeat fasciclin 1 (FAS1) domains of human periostin play crucial roles in promoting tumor metastasis and progression via interaction with cell surface integrins. Among four FAS1 domains of human periostin, the fourth FAS1 domain (FAS1-IV) was prepared for NMR study, since only FAS1-IV was highly soluble, and showed a well-dispersed 2D 1H-15N HSQC spectrum. Here, we report nearly complete backbone and side chain resonance assignments and a secondary structural analysis of the FAS1-IV domain as first steps toward the structure determination of FAS1-IV of human periostin.
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页码：95 / 98
页数：3
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