Piscidin-1 Induces Apoptosis via Mitochondrial Reactive Oxygen Species-Regulated Mitochondrial Dysfunction in Human Osteosarcoma Cells

被引：0
作者：
Meng-Hsuan Cheng
Chieh-Yu Pan
Nan-Fu Chen
San-Nan Yang
Shuchen Hsieh
Zhi-Hong Wen
Wu-Fu Chen
Jin-Wei Wang
Wen-Hsien Lu
Hsiao-Mei Kuo
机构：
[1] Division of Pulmonary and Critical Care Medicine,
[2] Department of Internal Medicine,undefined
[3] Kaohsiung Medical University Hospital,undefined
[4] School of Medicine,undefined
[5] College of Medicine,undefined
[6] Kaohsiung Medical University,undefined
[7] Department of Respiratory Therapy,undefined
[8] College of Medicine,undefined
[9] Kaohsiung Medical University,undefined
[10] Department and Graduate Institute of Aquaculture,undefined
[11] National Kaohsiung University of Science and Technology,undefined
[12] Division of Neurosurgery,undefined
[13] Department of Surgery,undefined
[14] Kaohsiung Armed Forces General Hospital,undefined
[15] Department of Neurological Surgery,undefined
[16] Tri-Service General Hospital,undefined
[17] National Defense Medical Center,undefined
[18] Department of Internal Medicine,undefined
[19] E-DA Hospital and College of Medicine,undefined
[20] I-SHOU University,undefined
[21] Department of Chemistry,undefined
[22] National Sun Yat-sen University,undefined
[23] Department of Marine Biotechnology and Resources,undefined
[24] National Sun Yat-sen University,undefined
[25] Doctoral Degree Program in Marine Biotechnology,undefined
[26] Academia Sinica,undefined
[27] Department of Neurosurgery,undefined
[28] Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine,undefined
[29] Department of Neurosurgery,undefined
[30] Xiamen Chang Gung Hospital,undefined
[31] Department of Orthopedic,undefined
[32] Kaohsiung Armed Forces General Hospital,undefined
[33] Department of Orthopedic,undefined
[34] Feng Yuan Hospital of the Ministry of Health,undefined
[35] Center for Neuroscience,undefined
[36] National Sun Yat-sen University,undefined
来源：
Scientific Reports | / 10卷
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摘要：
Osteosarcoma (OSA) is the most common type of cancer that originates in the bone and usually occurs in young children. OSA patients were treated with neoadjuvant chemotherapy and surgery, and the results were disappointing. Marine antimicrobial peptides (AMPs) have been the focus of antibiotic research because they are resistant to pathogen infection. Piscidin-1 is an AMP from the hybrid striped bass (Morone saxatilis × M. chrysops) and has approximately 22 amino acids. Research has shown that piscidin-1 can inhibit bacterial infections and has antinociception and anti-cancer properties; however, the regulatory effects of piscidin-1 on mitochondrial dysfunction in cancer cells are still unknown. We aimed to identify the effects of piscidin-1 on mitochondrial reactive oxygen species (mtROS) and apoptosis in OSA cells. Our analyses indicated that piscidin-1 has more cytotoxic effects against OSA cells than against lung and ovarian cancer cells; however, it has no effect on non-cancer cells. Piscidin-1 induces apoptosis in OSA cells, regulates mtROS, reduces mitochondrial antioxidant manganese superoxide dismutase and mitochondrial transmembrane potential, and decreases adenosine 5′-triphosphate production, thus leading to mitochondrial dysfunction and apoptosis. The mitochondrial antioxidant, mitoTempo, reduces the apoptosis induced by piscidin-1. Results suggest that piscidin-1 has potential for use in OSA treatment.
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