The deletion of Ffh in Escherichia coli induces adverse physiological and morphological changes

被引：0
作者：
Dong H.J. [1 ]
Shen X.L. [2 ]
Jiang J.Y. [1 ]
Li Y.Q. [2 ]
机构：
[1] Institute of Drug Research, Lunan Pharmaceutical Company
[2] College of Life Science, Zhejiang University
来源：
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology | 2009年 / 3卷 / 2期
基金：
中国国家自然科学基金;
关键词：
Escherichia coli; Ffh; Membrane proteins;
D O I：
10.1134/S1990747809020093
中图分类号：
学科分类号：
摘要：
The Escherichia coli Ffh protein is homologous to the SRP54 subunit of the eukaryotic signal recognition particle (SRP) that is involved in targeting and translocation of membrane proteins. The functions of Ffh in E. coli were investigated using the mutant with the Ffh deficiency. The mutant showed lower growth rate at 30°C and rapidly lost viability at the non-permissive temperature of 42°C. In addition, the amount of the total membrane proteins decreased sharply in the mutant. The mutant cells cultured at either 30 or 42°C appeared to have an elongated shape as compared to the wild type cells. Transmission electron microscopy revealed that the membrane layer of the mutant cells was thinner than that of the wild type cells. © Pleiades Publishing, Ltd. 2009.
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收藏
页码：163 / 167
页数：4
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