Preparation and Performance Research on Glutathione Molecularly Imprinted Polymers

Methacrylic acid and ethylene glycol dimethacrylate were respectively used as functional monomer and cross-linker to synthesize molecularly imprinted polymers (MIPs), for adsorption of glutathione (GSH), named GSH-MIPs. Evaluation of various polymers by static experiment indicated the optimum ratio of template to functional monomer was 1:4; the optimal concentration and pH of loading buffer were 5.00–6.50 mmol L−1 and 5.0, respectively. By Scatchard analysis, the GSH binding site was found in GSH-MIPs, with Kd and Qmax of 1.96 mmol L−1 and 47.19 μmol g−1, respectively. The method validated the extraction of GSH from yeast cells samples with the enrichment rate of 76%.