Biological imaging of chemical bonds by stimulated Raman scattering microscopy

被引：0
作者：
Fanghao Hu
Lixue Shi
Wei Min
机构：
[1] Columbia University,Department of Chemistry
[2] Columbia University,Kavli Institute for Brain Science
来源：
Nature Methods | 2019年 / 16卷
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摘要：
All molecules consist of chemical bonds, and much can be learned from mapping the spatiotemporal dynamics of these bonds. Since its invention a decade ago, stimulated Raman scattering (SRS) microscopy has become a powerful modality for imaging chemical bonds with high sensitivity, resolution, speed and specificity. We introduce the fundamentals of SRS microscopy and review innovations in SRS microscopes and imaging probes. We highlight examples of exciting biological applications, and share our vision for potential future breakthroughs for this technology.
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页码：830 / 842
页数：12
相关论文
共 284 条
[1]  
Ploetz E(2007)Femtosecond stimulated Raman microscopy Appl. Phys. B 87 389-393
[2]  
Laimgruber S(2008)Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy Science 322 1857-1861
[3]  
Berner S(2009)Vibrational imaging based on stimulated Raman scattering microscopy New J. Phys. 11 033026-3658
[4]  
Zinth W(2009)Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy Opt. Express 17 3651-328
[5]  
Gilch P(1967)The stimulated Raman effect Am. J. Phys. 35 989-1432
[6]  
Freudiger CW(1977)Sensitivity limitations for CW stimulated Raman-spectroscopy Opt. Commun. 22 323-488
[7]  
Nandakumar P(1979)Surface vibrational spectroscopy using stimulated Raman-scattering IEEE J. Quantum Elect. 15 1418-530
[8]  
Kovalev A(2007)Femtosecond stimulated Raman spectroscopy Annu. Rev. Phys. Chem. 58 461-305
[9]  
Volkmer A(2011)Coherent nonlinear optical imaging: beyond fluorescence microscopy Annu. Rev. Phys. Chem. 62 507-5094
[10]  
Ozeki Y(2015)Vibrational spectroscopic imaging of living systems: an emerging platform for biology and medicine Science 350 aaa8870-1370
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