Isolation and Characterization of ACC Deaminase Gene from Two Plant Growth-Promoting Rhizobacteria

被引：0
作者：
Venkadasamy Govindasamy
Murugesan Senthilkumar
Kishore Gaikwad
Kannepalli Annapurna
机构：
[1] Indian Agricultural Research Institute,Division of Microbiology
[2] IARI,National Research Centre on Plant Biotechnology
来源：
Current Microbiology | 2008年 / 57卷
关键词：
Polymerase Chain Reaction Amplification; Pseudomonas Fluorescens; Pseudomonas Stutzeri; Nonspecific Band; acdS Gene;
D O I：
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中图分类号：
学科分类号：
摘要：
Lowering of plant ethylene by deamination of its immediate precursor 1-aminocyclopropane-1-carboxylate (ACC) is a key trait found in many rhizobacteria. We isolated and screened bacteria from the rhizosphere of wheat for their ACC-degrading ability. The ACC deaminase gene (acdS) isolated from two bacterial isolates through PCR amplification was cloned and sequenced. Nucleotide sequence alignment of these genes with previously reported genes of Pseudomonas sp. strain ACP and Enterobactercloacae strain UW4 showed variation in their sequences. In the phylogenetic analysis, distinctness of these two genes was observed as a separate cluster. 16S rDNA sequencing of two isolates identified them to be Achromobacter sp. and Pseudomonasstutzeri.
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页码：312 / 317
页数：5
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