Development and characterization of 14 microsatellite loci from an enriched genomic library of Eucalyptus camaldulensis Dehnh

被引：0
作者：
Janete Motta da Silva
Adna Cristina Barbosa de Sousa
Anete Pereira de Souza
Edson Seizo Mori
Miguel Luiz Menezes Freitas
Alexandre Magno Sebbenn
Mario Luiz Teixeira de Moraes
机构：
[1] Universidade Estadual Paulista (UNESP),Faculdade de Engenharia
[2] Universidade Estadual de Campinas (Unicamp),Centro de Biologia Molecular e Engenharia Genética
[3] Universidade Estadual de Campinas (Unicamp),Departamento de Biologia Vegetal
[4] Universidade Estadual Paulista (UNESP),Faculdade de Ciências Agronômicas
[5] Instituto Florestal de São Paulo,Departamento de Fitotecnia, Tecnologia de Alimentos e Sócio
[6] Universidade Estadual Paulista (UNESP),Economia, Faculdade de Engenharia de Ilha Solteira
来源：
Conservation Genetics Resources | 2009年 / 1卷
关键词：
Conservation genetic; Hardwoods; Molecular marker; Microsatellites; Cross-species amplification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Eucalyptus camaldulensis Dehnh is an Australian tree species which occurs in various climatic and environmental conditions and show large genetic diversity. Twenty five microsatellite markers were developed from a CT8–GT8 enriched genomic library of E. camaldulensis. The number of alleles ranged from 4 to 13 (average of 8). The polymorphism information content (PIC) and the discriminating power (D) of each primer ranged from 0.37 to 0.88 (average of 0.72) and 0.48 to 0.99 (average of 0.84), respectively. The observed and expected heterozygosities ranged from 0.28 to 0.84 and 0.25 to 0.90, respectively. Four loci showed statistically significant from Hardy-Weinberg equilibrium after Bonferroni correction (P, (5%) < 0.0038). All polymorphic markers were tested for cross-amplification in 25 different Eucalyptus species. Those microsatellite loci will be useful to investigate questions of genetic diversity and structure, gene flow, mating system and ex situ genetic conservation of E. camaldulensis.
引用
收藏
相关论文
共 46 条
  • [1] Billotte N(1999)Microsatellite enriched libraries: applied methodology for the development of SSR markers in tropical crops Fruits 54 277-288
  • [2] Lagoda PJR(1998)Development, characterization and mapping of microsatellite markers in Theor Appl Genet 97 816-827
  • [3] Risterucci AM(2002) and Heredity 88 402-412
  • [4] Baurens FC(2009)Nuclear RFLP variation in Tree Genet Genomes 5 189-210
  • [5] Brondani RPV(2000) Dehnh from northern Australia Plant Sci 155 161-168
  • [6] Brondani C(2001)Congruence between environmental parameters, morphology and genetic structure in Australia’s most widely distributed eucalypt, Plant Mol Biol Rep 19 299-306
  • [7] Tarchini R(1986)Characterization of microsatellite markers from sugarcane ( Aust For 49 16-27
  • [8] Grattapaglia D(1991) sp.), a highly polyploidy species Nucleic Acids Res 19 4008-15
  • [9] Butcher PA(1990)Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining Focus 12 13-486
  • [10] Otero A(1995)River regulation and associate forest management problems in the River Murray red gum forests J Hered 86 485-1106
12345