A mutation in the gene for polynucleotide kinase of bacteriophage T4 K10 affects mRNA processing

被引：0
作者：
Živilė Strazdaitė-Žielienė
Aurelija Zajančkauskaitė
Laura Kalinienė
Rolandas Meškys
Lidija Truncaitė
机构：
[1] Institute of Biochemistry of Vilnius University,Department of Molecular Microbiology and Biotechnology
来源：
Archives of Virology | 2014年 / 159卷
关键词：
G14D Mutation; Progeny Phage; Secondary Cleavage; Mutant Phage; supF Gene;
D O I：
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摘要：
The bacteriophage T4 insertion-substitution (I/S) vector system has become one of the most important tools for the introduction of site-directed mutations into the T4 genome. In this study, we show that the I/S phage T4 K10 carries two point mutations within the gene for polynucleotide kinase pseT, resulting in amino acid substitutions G14D and R229H. The G14D mutation impairs 5′-kinase activity in vivo as well as in vitro and leads to diminished processing at secondary sites of several RegB-cleaved transcripts.
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页码：327 / 331
页数：4
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