Reversed-phase liquid chromatography and argentation chromatography of the minor capsaicinoids

被引：0
作者：
Robert Q. Thompson
Karen W. Phinney
Lane C. Sander
Michael J. Welch
机构：
[1] Oberlin College,Department of Chemistry
[2] National Institute of Standards and Technology,Chemical Science and Technology Laboratory
来源：
Analytical and Bioanalytical Chemistry | 2005年 / 381卷
关键词：
Capsaicinoids; Chili pepper; Reversed-phase liquid chromatography; Argentation Chromatography; Silver;
D O I：
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中图分类号：
学科分类号：
摘要：
An investigation of the liquid chromatography of the minor capsaicinoids in a commercial capsaicinoid mixture is reported. Twelve stationary phases including C8, C18, C30, phenyl, and cation-exchange chemistries were examined in combination with isocratic aqueous methanol and aqueous acetonitrile mobile phases. A phenyl stationary phase and aqueous acetonitrile mobile phase baseline-resolved 7 of 11 capsaicinoids, and selected ion chromatograms (LC–ESI-MS) demonstrated this was the most effective reversed-phase separation. Argentation chromatography with an alkyl or phenyl column and aqueous silver nitrate–methanol mobile phase revealed the presence of the 6-ene-8-methyl and 6-ene-9-methyl homocapsaicin isomers and the absence of 7-ene-9-methyl homocapsaicin. A mixed phenyl–cation-exchange stationary phase (charged with silver ion) enabled unique and useful separations of the capsaicinoids.
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页码：1432 / 1440
页数：8
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