Specificity of a polymerase chain reaction assay of a target sequence on the 31-kilodalton Brucella antigen DNA used to diagnose human brucellosis

被引：52

作者：

Casañas M.C. ^{[1
]}

Queipo-Ortuño M.I. ^{[2
]}

Rodriguez-Torres A. ^{[3
]}

Orduña A. ^{[3
]}

Colmenero J.D. ^{[4
]}

Morata P. ^{[2
]}

机构：

[1] Microbiology Service, Carlos Haya Hospital, Malaga

[2] Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Malaga

[3] Department of Microbiology, University Hospital, Faculty of Medicine, Valladolid

[4] Infectious Diseases Unit, Department of Internal Medicine, 29010 Malaga, Camino de Antequera s/n

来源：

European Journal of Clinical Microbiology and Infectious Diseases | 2001年 / 20卷 / 2期

关键词：

Polymerase Chain Reaction; Clinical Study; Target Sequence; Field Strain; Polymerase Chain Reaction Assay;

D O I：

10.1007/PL00011242

中图分类号：

学科分类号：

摘要：

The aim of this study was to evaluate the specificity of a polymerase chain reaction assay for detecting Brucella DNA using primers specific for the amplification of a 223 bp region of the sequence encoding a 31 kDa immunogenic Brucella abortus protein (BCSP31). DNA from all Brucella strains, including type, reference, vaccine and field strains, were correctly amplified. With the exception of Ochrobactrum spp., no other amplification was detected with a broad panel of microorganisms serologically or phylogenetically related to Brucella spp. This very good degree of specificity, together with its high yield demonstrated in previous clinical studies, confirms that this polymerase chain reaction assay could be a useful tool for the diagnosis of human brucellosis.

引用

页码：127 / 131

页数：4

共 17 条

[1]

Matyas Z., Fujikura T., Brucellosis as a world problem, Developments in Biological Standardization, 56, pp. 3-20, (1984)

[2]

Colmenero J.D., Reguera J.M., Martos F., Sanchez-Mora D., Delgado M., Causse M., Martin-Farfan A., Juarez C., Complications associated with Brucella melitensis infection: A study of 530 cases, Medicine (Baltimore), 75, pp. 195-211, (1996)

[3]

Yagupsky P., Detection of Brucella melitensis by BACTEC NR660 blood culture system, Journal of Clinical Microbiology, 32, pp. 1899-1901, (1994)

[4]

Ariza J., Pellicer T., Pallares R., Foz A., Gudiol F., Specific antibody profile in human brucellosis, Clinical Infectious Diseases, 14, pp. 131-140, (1992)

[5]

Leal-Klevezas D., Martinez-Vazquez I.O., Lopez-Merino A., Martinez-Soriano J.P., Single-step PCR for detection of Brucella spp. from blood and milk of infected animals, Journal of Clinical Microbiology, 33, pp. 3087-3090, (1995)

[6]

Matar G.M., Khneisser I.A., Abdelnoor A.M., Rapid laboratory confirmation of human brucellosis by PCR analysis of a target sequence on the 31-kilodalton Brucella antigen DNA, Journal of Clinical Microbiology, 34, pp. 477-478, (1996)

[7]

Baily G.G., Krahn J.B., Drasar B.S., Stoker N.G., Detection of Brucella melitensis and Brucella abortus by DNA amplification, Journal of Tropical Medicine and Hygiene, 95, pp. 271-275, (1992)

[8]

Queipo-Ortuno M.I., Morata P., Ocon P., Manchado P., Colmenero J.D., Rapid diagnosis of human brucellosis by peripheral blood PCR assay, Journal of Clinical Microbiology, 35, pp. 2927-2930, (1997)

[9]

Morata P., Queipo-Ortuno M.I., Reguera J.M., Garcia-Ordonez M.A., Pichardo C., Colmenero J.D., Posttreatment follow-up of brucellosis by PCR assay, Journal of Clinical Microbiology, 37, pp. 4163-4166, (1999)

[10]

Miller S.A., Dykes D.D., Polesky H.F., A simple salting out procedure for extracting DNA from human nucleated cells, Nucleic Acids Research, 16, (1988)

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