In vitro anti-Trichomonas vaginalis activity of Pistacia lentiscus mastic and Ocimum basilicum essential oil

被引：26

作者：

Ezz Eldin H.M. ^{[1
]}

Badawy A.F. ^{[1
]}

机构：

[1] Medical Parasitology Department, Ain Shams University, Cairo

来源：

Journal of Parasitic Diseases | 2015年 / 39卷 / 3期

关键词：

Ocimum basilicum oil; Pistacia lentiscus mastic; Trichomonas vaginalis; Trichomoniasis;

D O I：

10.1007/s12639-013-0374-6

中图分类号：

学科分类号：

摘要：

Trichomonas vaginalis is a protozoan parasite that causes trichomoniasis; a cosmopolitan sexually transmitted disease. Metronidazole is the drug of choice for T. vaginalis infections. The increase in metronidazole resistant parasites and undesirable side effects of this drug makes the search for an alternative a priority for the management of trichomoniasis. Pistacia lentiscus mastic and Ocimum basilicum oil are known for their antibacterial, antifungal, antiviral and antiprotozoal effects. The present study was carried out to investigate the in vitro effects of P. lentiscus mastic and O. basilicum oil on T. vaginalis trophozoites. The effects of different concentrations of P. lentiscus mastic (15, 10 and 5 mg/ml) and different concentrations of O. basilicum oil (30, 20 and 10 μg/ml) on multiplication of trophozoites at different time points (after 24, 48, 72 and 96 h) were determined, also morphological changes were reported by transmission electron microscopy (TEM). The results showed that both plants caused an inhibition of growth of T. vaginalis trophozoites. The minimal lethal concentration of P. lentiscus mastic was 15 mg/ml after 24 h incubation, 10 mg/ml after 48 h and 5 mg/ml after 96 h. The minimal lethal concentration of O. basilicum oil was 30 μg/ml after 24 h incubation, 20 μg/ml after 48 h and 10 μg/ml after 96 h. TEM study of trophozoites treated by P. lentiscus mastic or by O. basilicum oil showed considerable damage of the membrane system of the trophozoites, and extensive vacuolization of the cytoplasm. These results highly suggest that P. lentiscus mastic and O.basilicum oil may be promising phytotherapeutic agents for trichomoniasis treatment. © 2013, Indian Society for Parasitology.

引用

页码：465 / 473

页数：8

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