Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum

被引：0
|
作者：
Priti N. Chaudhari
Bhushan L. Chaudhari
Sudhir B. Chincholkar
机构：
[1] North Maharashtra University,School of Life Sciences
来源：
Biotechnology Letters | 2010年 / 32卷
关键词：
Androsta-1,4-diene-3,17-dione; Biotransformation; Cholesterol;
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学科分类号：
摘要：
Cholesterol oxidase activity was studied during biotransformation of cholesterol to androsta-1,4-diene-3,17-dione (ADD) by Chryseobacterium gleum. Spent LB media, containing cholesterol (3 mM≈1 g l−1) where the bacterium was grown for 24 h, at 30°C with constant shaking at 120 rpm, had the highest enzyme activity (167 U mg−1). The growing cells produced 0.076 g ADD from 1 g cholesterol l−1.
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页码：695 / 699
页数：4
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