Immunophenotypic and gene expression analysis of monoclonal B-cell lymphocytosis shows biologic characteristics associated with good prognosis CLL

被引:0
作者
M C Lanasa
S D Allgood
S L Slager
S S Dave
C Love
G E Marti
N E Kay
C A Hanson
K G Rabe
S J Achenbach
L R Goldin
N J Camp
B K Goodman
C M Vachon
L G Spector
L Z Rassenti
J F Leis
J P Gockerman
S S Strom
T G Call
M Glenn
J R Cerhan
M C Levesque
J B Weinberg
N E Caporaso
机构
[1] Duke University Medical Center,Department of Medicine
[2] Mayo Clinic College of Medicine,Department of Health Sciences Research
[3] Cellular and Tissue Therapy Branch,Department of Medicine
[4] CBER,Department of Pathology
[5] OCTGC,Department of Biomedical Informatics
[6] Food and Drug Administration,Department of Pathology
[7] Mayo Clinic College of Medicine,Department of Pediatrics
[8] Mayo Clinic College of Medicine,Department of Medicine
[9] Genetic Epidemiology Branch,Department of Medicine
[10] National Cancer Institute,Department of Epidemiology
[11] University of Utah,Department of Medicine
[12] Duke University Medical Center,Department of Medicine
[13] University of Minnesota,Department of Medicine
[14] Moores UCSD Cancer Center,undefined
[15] Mayo Clinic College of Medicine,undefined
[16] MD Anderson Cancer Center,undefined
[17] University of Utah,undefined
[18] University of Pittsburgh Medical Center,undefined
[19] Durham Veterans Affairs Medical Center,undefined
来源
Leukemia | 2011年 / 25卷
关键词
chronic lymphocytic leukemia; monoclonal B lymphocytosis; immunophenotype; B-cell immunology; gene expression profiling;
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中图分类号
学科分类号
摘要
Monoclonal B-cell lymphocytosis (MBL) is a hematologic condition wherein small B-cell clones can be detected in the blood of asymptomatic individuals. Most MBL have an immunophenotype similar to chronic lymphocytic leukemia (CLL), and ‘CLL-like’ MBL is a precursor to CLL. We used flow cytometry to identify MBL from unaffected members of CLL kindreds. We identified 101 MBL cases from 622 study subjects; of these, 82 individuals with MBL were further characterized. In all, 91 unique MBL clones were detected: 73 CLL-like MBL (CD5+CD20dimsIgdim), 11 atypical MBL (CD5+CD20+sIg+) and 7 CD5neg MBL (CD5negCD20+sIgneg). Extended immunophenotypic characterization of these MBL subtypes was performed, and significant differences in cell surface expression of CD23, CD49d, CD79b and FMC-7 were observed among the groups. Markers of risk in CLL such as CD38, ZAP70 and CD49d were infrequently expressed in CLL-like MBL, but were expressed in the majority of atypical MBL. Interphase cytogenetics was performed in 35 MBL cases, and del 13q14 was most common (22/30 CLL-like MBL cases). Gene expression analysis using oligonucleotide arrays was performed on seven CLL-like MBL, and showed activation of B-cell receptor associated pathways. Our findings underscore the diversity of MBL subtypes and further clarify the relationship between MBL and other lymphoproliferative disorders.
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页码:1459 / 1466
页数:7
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