Expression of two human acyl-CoA:diacylglycerol acyltransferase isozymes in yeast and selectivity of microbial inhibitors toward the isozymes

被引：0
|
作者：
Junji Inokoshi
Kyosuke Kawamoto
Yoichi Takagi
Maki Matsuhama
Satoshi Ōmura
Hiroshi Tomoda
机构：
[1] School of Pharmacy,
[2] Kitasato University,undefined
[3] Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences,undefined
[4] Kitasato University,undefined
来源：
The Journal of Antibiotics | 2009年 / 62卷
关键词：
acyl-CoA:diacylglycerol acyltransferase; amidepsine; DGAT; isozyme; roselipin; triacylglycerol; xanthohumol;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two isozymes for human acyl-coenzyme A:diacylglycerol acyltransferase (DGAT), DGAT1 and DGAT2, were independently expressed in DGAT-deficient Saccharomyces cerevisiae to establish DGAT1- and DGAT2-S. cerevisiae. The selectivity of DGAT inhibitors of natural origin towards the isozymes was assessed in enzyme assays using the microsomal fractions prepared from DGAT1- and DGAT2-S. cerevisiae. Amidepsines and xanthohumol inhibited DGAT1 and DGAT2 with similar potency, whereas roselipins were found to inhibit DGAT2 selectively.
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页码：51 / 54
页数：3
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