High-resolution 3D analysis of mouse small-intestinal stroma

被引：0
作者：
Jeremiah Bernier-Latmani
Tatiana V Petrova
机构：
[1] Ludwig Institute for Cancer Research and Institute of Pathology,Department of Fundamental Oncology
[2] Centre Hospitalier Universitaire Vaudois (CHUV) and University of Lausanne (UNIL),undefined
[3] Swiss Institute for Experimental Cancer Research (ISREC),undefined
[4] School of Life Sciences. Swiss Federal Institute of Technology Lausanne (EPFL),undefined
来源：
Nature Protocols | 2016年 / 11卷
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学科分类号：
摘要：
This protocol describes how to perform whole-mount immunostaining and imaging on adult mouse small-intestinal villi. All gut cell types can be seen at high resolution and in 3D without the need for image reconstruction.
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页码：1617 / 1629
页数：12
相关论文
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[1]  
Gerhardt H(2003)VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia J. Cell Biol. 161 1163-1177
[2]  
Pitulescu ME(2010)Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice Nat. Protoc. 5 1518-1534
[3]  
Schmidt I(2009)FOXC2 controls formation and maturation of lymphatic collecting vessels through cooperation with NFATc1 J. Cell Biol. 185 439-457
[4]  
Benedito R(2015)State-of-the-art methods for evaluation of angiogenesis and tissue vascularization: a scientific statement from the American Heart Association Circ. Res. 116 e99-132
[5]  
Adams RH(2014)Lymphangiogenic factors, mechanisms, and applications J. Clin. Invest. 124 878-887
[6]  
Norrmén C(2011)Basic and therapeutic aspects of angiogenesis Cell 146 873-887
[7]  
Simons M(2010)Homeostasis and inflammation in the intestine Cell 140 859-870
[8]  
Zheng W(2006)VEGF-dependent plasticity of fenestrated capillaries in the normal adult microvasculature Am. J. Physiol. Heart Circ. Physiol. 290 H560-H576
[9]  
Aspelund A(2013)Anti-VEGF- and anti-VEGF receptor-induced vascular alteration in mouse healthy tissues Proc. Natl. Acad. Sci. USA 110 12018-12023
[10]  
Alitalo K(2002)Developmental regulation of intestinal angiogenesis by indigenous microbes via Paneth cells Proc. Natl. Acad. Sci. USA 99 15451-15455
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