Ligustilide attenuates hyperalgesia in an inflammatory pain rat model: involvement of spinal ERK/MAPK pathway and proinflammatory cytokines

被引：0
作者：
Chang-Cheng Lyu
Shu-Juan Shu
机构：
[1] Zhejiang University,Department of Anesthesiology, The Women’s Hospital, School of Medicine
[2] Zhejiang University,Department of Obstetrics, The Women’s Hospital, School of Medicine
来源：
Medicinal Chemistry Research | 2018年 / 27卷
关键词：
Ligustilide; Pain; ERK1/2; ERK5; IL-1β; TNF-α;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The aim of this study is to examine the analgesic effect of ligustilide in a rat model of complete Freund’s adjuvant (CFA)-induced inflammatory pain, and to investigate whether the spinal ERK/MAPK pathway and the subsequent activation of proinflammatory cytokines were involved in ligustilide’s anti-nociceptive effect. Adult male Sprague–Dawley rats were divided into three groups: Control+Vehicle, CFA+Vehicle, CFA+Ligustilide. The ligustilide was administrated intragastrically on 1 day before CFA injection, 0, 4, 8 h after CFA injection, and 1 day after injection. Paw withdrawal latency (PWL) and paw withdrawal threshold (PWT) were measured. The change of the activation of spinal ERK1/2 and ERK5, and the level of IL-1β and TNF-α were detected by western blotting and ELISA on day 2 after CFA injection. The behavior results showed that consecutive intragastric administration of ligustilide alleviated the CFA-induced hyperalgesia. Ligustilide inhibited the CFA-induced increased activation of spinal ERK1/2 and ERK5, as well as reducing the increased production of IL-1β and TNF-α.
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页码：1292 / 1297
页数：5
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