Regulatory T cells but not T helper 17 cells are modulated in an animal model of Graves’ hyperthyroidism

被引：0
作者：
Jin Zhou
Mei Bi
Chenling Fan
Xizhu Song
Rong Yang
Shujie Zhao
Li Li
Yushu Li
Weiping Teng
Zhongyan Shan
机构：
[1] The First Hospital of China Medical University,The Endocrine Institute of China Medical University
[2] The First Hospital of China Medical University,The Liaoning Provincial Key Laboratory of Endocrine diseases
[3] The First Hospital of China Medical University,Department of Endocrinology and Metabolism
来源：
Clinical and Experimental Medicine | 2012年 / 12卷
关键词：
Th17; Treg; Graves’ hyperthyroidism; Th17/Treg ratio;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Graves’ disease (GD) involves auto-immunity against thyroid cell antigens, but the reasons for the induction of auto-immunity are uncertain. We wished to investigate the role of T helper 17 (Th17) and regulatory T cells (Treg) in a mouse model of Graves’ hyperthyroidism. The model was generated by immunizing mice with adenovirus expressing the autoantigen thyroid-stimulating hormone receptor (Ad-TSHR289). The frequencies of splenic Th17 and Treg were determined by flow cytometry. The levels of interleukin-17(IL-17), forkhead box P3 (Foxp3), and orphan retinoic acid nuclear receptor (RORγt) mRNA were determined by real-time PCR. The number of CD4+CD25+Foxp3+ T lymphocyte was significantly reduced in the Ad-TSHR289 group compared with the Ad-control (P < 0.05). mRNA level for Foxp3 was less abundant in Ad-TSHR289 group compared with Ad-control (P < 0.05). However, CD4+IL-17+ T-cell subpopulation and expression of RORγt mRNA did not differ significantly between Ad-TSHR289 and Ad-control groups (P > 0.05). Nevertheless, in Ad-TSHR289 group, a profound increase in the Th17/Treg ratios was found. The present study demonstrates that Th17 is not involved in promoting Graves’ hyperthyroidism, while Treg and the ratio of Th17/Treg might play a role in the pathogenesis of Graves’ hyperthyroidism.
引用
收藏
页码：39 / 46
页数：7
相关论文
共 118 条
  • [1] McLachlan SM(2005)Insight into Graves’ hyperthyroidism from animal models Endocr Rev 26 800-832
  • [2] Nagayama Y(1998)The thyrotropin (TSH) receptor: interaction with TSH and autoantibodies Endocr Rev 19 673-716
  • [3] Rapoport B(2009)The thyroid and autoimmunity Rom J Intern Med 47 207-215
  • [4] Rapoport B(2008)CD4 T cells: fates, functions, and faults Blood 112 1557-1569
  • [5] Chazenbalk GD(2010)Graves’ disease and gene polymorphism of TNF-α, IL-2, IL-6, IL-12, and IFN-γ Endocrine 37 344-348
  • [6] Jaume JC(2007)Levels of cytokines and thyroid autoantibodies in Omani patients with Graves’ disease Br J Biomed Sci 64 164-167
  • [7] McLachlan SM(2007)Animal models of Graves’ hyperthyroidism Thyroid 17 981-988
  • [8] Lichiardopol C(2007)A brief history of T(H)17, the first major revision in the T(H)1/T(H)2 hypothesis of T cell-mediated tissue damage Nat Med 13 139-145
  • [9] Moţa M(2003)Suppression of immune induction of collagen-induced arthritis in IL-17-deficient mice J Immunol 171 6173-6177
  • [10] Zhu J(2005)Blocking adhesion molecules as therapy for multiple sclerosis: natalizumab Nat Rev Drug Discov 4 510-518
12345678910