Protection of peroxynitrite-induced DNA damage by dietary antioxidants

被引：0
作者：
Hye Kyung Moon
Eun Sun Yang
Jeen Woo Park
机构：
[1] Kyungpook National University,School of Life Sciences and Biotechnology and Agro
来源：
Archives of Pharmacal Research | 2006年 / 29卷
关键词：
Dietary antioxidants; Peroxynitrite; DNA damage;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The present study was undertaken to test the hypothesis that dietary antioxidants protect DNA damage induced by peroxynitrite, a potent physiological inorganic toxin. The present study showed that dietary antioxidants such as (−)-epigallocatechin, gallate, quercerin, rutin, resveratrol, and ursolic acid inhibit single strand breaks in supercoiled plasmid DNA induced by 3-morpholinosyndromine N-ethylcarbamide (SIN-1), a generator of peroxynitrite, through the reaction between nitric oxide and superoxide anion. The formation of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) in calf thymus DNA by SIN-1 was also inhibited by dietary antioxidants. When U937 cells were incubated with 1 mM SIN-1 bolus, a significant increase of 8-OH-dG level was observed. However, oxidative DNA damage was significantly lower in the cells pre-treated with dietary antioxidants when cells were exposed to SIN-1.
引用
收藏
页码：213 / 217
页数：4
相关论文
共 69 条
  • [11] Beckman J. S.(1994)Evidence for nitric oxide-mediated oxidative damage in chronic inflammation. Nitrotyrosine in serum and synovial fluid from rheumatoid patients FEBS Lett. 350 9-12
  • [12] Beckman T. W.(1994)Agonist-induced peroxynitrite production from endothelial cells Arch. Biochem. Biophys. 310 352-359
  • [13] Chen J.(1992)Peroxynitrite, a cloaked oxidant formed by nitric oxide and superoxide Chem. Res. Toxicol. 5 834-842
  • [14] Marshall P. A.(1992)Lipid peroxidation products mediate the formation of 8-hydroxydeoxyguanosine in DNA Free Radic. Biol. Med. 12 245-250
  • [15] Freeman B. A.(1992)Generation of strand breaks and formation of 8-hydroxy-2′-deoxyguanosine in DNA by a thiol/Fe Arch. Biochem. Biophys. 12 245-250
  • [16] Beckman J. S.(1991)/O J. Biol. Chem. 266 4244-4250
  • [17] Carson M.(1996)-catalyzed oxidation system Arch. Biochem. Biophys. 333 482-488
  • [18] Smith C. D.(1991)Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide Nature 349 431-434
  • [19] Koppenol W. H.(1998)Trolox inhibits peroxynitrite-mediated oxidative stress and apoptosis in rat thymocytes Anal. Biochem. 255 20-31
  • [20] Carreras M. C.(2002)Insertion of specific base during DNA synthesis past the oxidation-damaged base 8-oxodG Free Radic. Biol. Med. 32 314-318
1234567