In-vivo intratissue ablation by nanojoule near-infrared femtosecond laser pulses

被引：0
作者：
Bao-Gui Wang
Iris Riemann
Harald Schubert
Karl-Juergen Halbhuber
Karsten Koenig
机构：
[1] University of Jena,Institute of Microscopic Anatomy (Anatomy II)
[2] Fraunhofer Institute of Biomedical Technology (IBMT),Institute of Laboratory Animals Science
[3] University of Jena,Faculty of Physics and Mechatronics
[4] Saarland University,undefined
[5] JenLab,undefined
来源：
Cell and Tissue Research | 2007年 / 328卷
关键词：
Ablation; Eye surgery; Myofibroblasts; Femtosecond laser; Nanoprocessing; Rabbit (New Zealand Albino);
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Non-invasive intratissue ablation was performed in the cornea of living rabbits by using 80 MHz near-infrared intense nanojoule femtosecond laser pulses. The intratissue surgical effect was induced by multiphoton absorption at a wavelength of 800 nm and was ascertained by histological examination. Highly precise intratissue ablation was obtained with no detrimental effects to the overlying or underlying layers. Activated keratocytes in the laser-treated corneas were detected with two-photon imaging postoperatively. Intratissue femtosecond laser ablation thus has potential as a effective technique in refractive surgery for the treatment of visual disorders.
引用
收藏
页码：515 / 520
页数：5
相关论文
共 148 条
[1]  
Baldwin HC(2002)Growth factors in corneal wound healing following refractive surgery: a review Acta Ophthalmol Scand 80 238-247
[2]  
Marshall J(2004)Microarray studies reveal macrophage-like function of stromal keratocytes in the cornea Invest Ophthalmol Vis Sci 45 3475-3484
[3]  
Chakravarti S(1990)Two-photon laser scanning fluorescence microscopy Science 248 73-76
[4]  
Wu F(2001)Proteoglycan expression during transforming growth factor beta-induced keratocyte-myofibroblast transdifferentiation J Biol Chem 276 44173-44178
[5]  
Vij N(1996)Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia Nat Med 2 449-456
[6]  
Roberts L(1996)Time-resolved observations of shock waves and cavitation bubbles generated by femtosecond laser pulses in corneal tissue and water Lasers Surg Med 19 23-31
[7]  
Joyce S(2003)Expression of type XVIII collagen during healing of corneal incisions and keratectomy wounds Invest Ophthalmol Vis Sci 44 78-85
[8]  
Denk W(2005)Cell nanosurgery using ultrashort laser pulses: applications to membrane surgery and cell isolation Lasers Surg Med 37 227-230
[9]  
Strickler JH(1999)Intracellular nanosurgery with near infrared femtosecond laser pulses Cell Mol Biol (Noisy-le-grand) 45 195-201
[10]  
Webb WW(2001)Nanodissection of human chromosomes with near-infrared femtosecond laser pulses Opt Lett 26 819-821
12345678910