Identification of a serotype-independent linear epitope of foot-and-mouth disease virus

被引:0
作者
Baolin Yang
Mingxia Wang
Wenming Liu
Zhiqiang Xu
Haiwei Wang
Decheng Yang
Wenge Ma
Guohui Zhou
Li Yu
机构
[1] Chinese Academy of Agricultural Sciences,Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute
[2] Xinjiang Academy of Animal Science,Institute of Veterinary Medicine
来源
Archives of Virology | 2017年 / 162卷
关键词
Foot-and-mouth disease virus; Monoclonal antibody; VP2; Epitope mapping;
D O I
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中图分类号
学科分类号
摘要
Foot-and-mouth disease (FMD), caused by foot-and-mouth disease virus (FMDV), is a highly contagious infectious disease that affects domestic and wild cloven-hoofed animals worldwide. VP2 is a structural protein of FMDV. In this study, an FMDV serotype-independent monoclonal antibody (MAb), 10B10, against the viral capsid protein VP2 was generated, and a series of GST fusion proteins expressing a truncated peptide of VP2 was subjected to Western blot analysis using MAb 10B10. Their results indicated that the peptide 8TLLEDRILT16 of VP2 is the minimal requirement of the epitope recognized by MAb 10B10. Importantly, this linear epitope was highly conserved among all seven serotypes of FMDV in a sequence alignment analysis. Subsequent alanine-scanning mutagenesis analysis revealed that the residues Thr8 and Asp12 of the epitope were crucial for MAb-10B10 binding. Furthermore, Western blot analysis also revealed that the MAb 10B10-directed epitope could be recognized by positive sera from FMDV-infected cattle. The discovery that MAb 10B10 recognizes a serotype-independent linear epitope of FMDV suggests potential applications for this MAb in the development of serotype-independent tests for FMDV.
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页码:3875 / 3880
页数:5
相关论文
共 122 条
[1]  
Bachrach HL(1968)Foot-and-mouth disease Annu Rev Microbiol 22 201-244
[2]  
Barteling SJ(2002)Development and performance of inactivated vaccines against foot and mouth disease Rev Sci Tech 21 577-588
[3]  
Curry S(1997)Dissecting the roles of VP0 cleavage and RNA packaging in picornavirus capsid stabilization: the structure of empty capsids of foot-and-mouth disease virus J Virol 71 9743-9752
[4]  
Fry E(2008)A novel computer algorithm improves antibody epitope prediction using affinity-selected mimotopes: a case study using monoclonal antibodies against the West Nile virus E protein Mol immunol 46 125-134
[5]  
Blakemore W(1999)Mutation rates among RNA viruses Proc Natl Acad Sci USA 96 13910-13913
[6]  
Abughazaleh R(2001)Type-independent detection of foot-and-mouth disease virus by monoclonal antibodies that bind to amino-terminal residues of capsid protein VP2 J Virol Methods 92 199-205
[7]  
Jackson T(2004)Foot-and-mouth disease Clin Microbiol Rev 17 465-493
[8]  
King A(2007)Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display J Mol Biol 365 196-210
[9]  
Lea S(2016)Identification of a conserved linear neutralizing epitope recognized by monoclonal antibody 9A9 against serotype A foot-and-mouth disease virus Arch Virol 161 2705-2716
[10]  
Newman J(2017)Identification of a conserved conformational epitope in the VP2 protein of foot-and-mouth disease virus Arch Virol 162 1877-1885