Recent trends in two-photon auto-fluorescence lifetime imaging (2P-FLIM) and its biomedical applications

被引：0
作者：
Harsh Ranawat
Sagnik Pal
Nirmal Mazumder
机构：
[1] Manipal Academy of Higher Education,Department of Biophysics, Manipal School of Life Sciences
来源：
Biomedical Engineering Letters | 2019年 / 9卷
关键词：
Two-photon excitation; Fluorescence lifetime imaging microscopy (FLIM); Nicotinamide adenine dinucleotide hydrogen (NADH); Flavin adenine dinucleotide (FAD);
D O I：
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学科分类号：
摘要：
Two photon fluorescence microscopy and the numerous technical advances to it have served as valuable tools in biomedical research. The fluorophores (exogenous or endogenous) absorb light and emit lower energy photons than the absorption energy and the emission (fluorescence) signal is measured using a fluorescence decay graph. Additionally, high spatial resolution images can be acquired in two photon fluorescence lifetime imaging (2P-FLIM) with improved penetration depth which helps in detection of fluorescence signal in vivo. 2P-FLIM is a non-invasive imaging technique in order to visualize cellular metabolic, by tracking intrinsic fluorophores present in it, such as nicotinamide adenine dinucleotide, flavin adenine dinucleotide and tryptophan etc. 2P-FLIM of these molecules enable the visualization of metabolic alterations, non-invasively. This comprehensive review discusses the numerous applications of 2P-FLIM towards cancer, neuro-degenerative, infectious diseases, and wound healing.
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页码：293 / 310
页数：17
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