Recent trends in two-photon auto-fluorescence lifetime imaging (2P-FLIM) and its biomedical applications

被引：0
作者：
Harsh Ranawat
Sagnik Pal
Nirmal Mazumder
机构：
[1] Manipal Academy of Higher Education,Department of Biophysics, Manipal School of Life Sciences
来源：
Biomedical Engineering Letters | 2019年 / 9卷
关键词：
Two-photon excitation; Fluorescence lifetime imaging microscopy (FLIM); Nicotinamide adenine dinucleotide hydrogen (NADH); Flavin adenine dinucleotide (FAD);
D O I：
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学科分类号：
摘要：
Two photon fluorescence microscopy and the numerous technical advances to it have served as valuable tools in biomedical research. The fluorophores (exogenous or endogenous) absorb light and emit lower energy photons than the absorption energy and the emission (fluorescence) signal is measured using a fluorescence decay graph. Additionally, high spatial resolution images can be acquired in two photon fluorescence lifetime imaging (2P-FLIM) with improved penetration depth which helps in detection of fluorescence signal in vivo. 2P-FLIM is a non-invasive imaging technique in order to visualize cellular metabolic, by tracking intrinsic fluorophores present in it, such as nicotinamide adenine dinucleotide, flavin adenine dinucleotide and tryptophan etc. 2P-FLIM of these molecules enable the visualization of metabolic alterations, non-invasively. This comprehensive review discusses the numerous applications of 2P-FLIM towards cancer, neuro-degenerative, infectious diseases, and wound healing.
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页码：293 / 310
页数：17
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[1]  
Becker W(2012)Fluorescence lifetime imaging—techniques and applications J Microsc 247 119-136
[2]  
Lin H(2003)Fluorescence lifetime-resolved pH imaging of living cells Cytometry 52A 77-89
[3]  
Herman P(1967)The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver Biochem J 103 514-527
[4]  
Lakowicz J(1979)Oxidation–reduction ratio studies of mitochondria in freeze-trapped samples. NADH and flavoprotein fluorescence signals J Biol Chem 254 4764-4771
[5]  
Williamson D(2009)Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment Clin Exp Metastasis 26 357-370
[6]  
Lund P(2007)Application of fluorescence resonance energy transfer resolved by fluorescence lifetime imaging microscopy for the detection of enterovirus 71 infection in cells J Biomed Opt 12 024016-1340
[7]  
Krebs H(2013)Investigation of tryptophan–NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopy J Biomed Opt 18 060501-482
[8]  
Chance B(2011)Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy Nat Protoc 6 1324-8773
[9]  
Schoener B(2007)In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia J Biomed Opt 12 024014-25126
[10]  
Oshino R(2015)Cellular autofluroscence detection through FLIM/FRET microscopy Curr Trends Opt Photon 129 471-1275
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