Performance and ease of use of a molecular point-of-care test for influenza A/B and RSV in patients presenting to primary care

被引：0
作者：
Jan Y Verbakel
Veerle Matheeussen
Katherine Loens
Mandy Kuijstermans
Herman Goossens
Margareta Ieven
Christopher C Butler
机构：
[1] University of Oxford,NIHR Community Healthcare MIC, Nuffield Department of Primary Care Health Sciences
[2] KU Leuven,Department of Public Health and Primary Care
[3] University of Oxford,Nuffield Department of Primary Care Health Sciences
[4] Antwerp University Hospital,Laboratory of Clinical Microbiology
[5] University of Antwerp,Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute (VAXINFECTIO)
来源：
European Journal of Clinical Microbiology & Infectious Diseases | 2020年 / 39卷
关键词：
Influenza; Point-of-care; Primary care; Ease-of-use; PCR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Annual influenza epidemics cause substantial morbidity and mortality, and the majority of patients with influenza-like illness present to primary care. Point-of-care influenza tests could support treatment decisions. It is critical to establish analytic performance of these platforms in real-life patient samples before uptake can be considered. We aimed to assess the analytical performance and ease of use of the cobas® Liat® PCR POCT in detecting influenza A/B and RSV in samples collected from patients with influenza-like illness in primary care. Sensitivity and specificity of the cobas® Liat® POCT are calculated in comparison with a commercial laboratory-based PCR test (Fast-Track Respiratory Pathogens 21 Plus kit (Fast-Track Diagnostics)). Samples with discordant results were analysed additionally by the RespiFinder 2Smart (PathoFinder) using an Extended Gold Standard (EGS). Acceptability was scored on a five-point Likert scale as well as a failure mode analysis of the cobas® Liat® POCT was performed. Nasal and oropharyngeal swabs were obtained from 140 children and nasopharyngeal swabs from 604 adults (744 patients). The cobas® Liat® POCT had a sensitivity and specificity of 100% (95% CI 99–100%) and 98.1% (95%CI 96.3–99%) for influenza A, 100% (95% CI 97.7–100%) and 99.7% (95%CI 98.7–99.9%) for influenza B and 100% (95% CI 87.1–100%) and 99.4% (95%CI 98.6–99.8%) for RSV, respectively. According to trained lab technicians, the cobas® Liat® POCT was considered easy-to-use, with a fast turn-around-time. Cobas® Liat® POCT is a promising decentralised test platform for influenza A/B and RSV in primary care as it provides fairly rapid results with excellent analytic performance. Point-of-care influenza tests could support treatment decisions in primary care. Cobas® Liat® POCT is a promising decentralised test platform for influenza A/B and RSV in primary care as it provides fairly rapid results with excellent analytic performance.
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页码：1453 / 1460
页数：7
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