Assessment of early blight (Alternaria solani) resistance in tomato using a droplet inoculation method

被引：53
作者：
Chaerani R. [1 ,3 ]
Groenwold R. [1 ]
Stam P. [2 ]
Voorrips R.E. [1 ]
机构：
[1] Plant Research International, Wageningen 6700 AA
[2] Laboratory of Plant Breeding, Department of Plant Sciences, Wageningen University, Wageningen
[3] Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor 16111
来源：
Journal of General Plant Pathology | 2007年 / 73卷 / 2期
关键词：
Alternaria solani; Early blight; Resistance; Screening method; Tomato;
D O I：
10.1007/s10327-006-0337-1
中图分类号：
学科分类号：
摘要：
A droplet inoculation method was used for evaluation of tomato resistance to early blight, a destructive foliar disease of tomato caused by Alternaria solani (Ellis and Martin) Sorauer. In this test method, leaflets are inoculated with small droplets of a spore suspension in either water or a 0.1% agar solution. Early blight resistance was evaluated based on lesion size. The droplet method better discriminated the level of resistance (P < 0.001) for a range of spore densities in comparison with the more commonly used spray inoculation method. Lesions generated by droplet inoculation at 7 days after inoculation ranged from small flecks to almost complete blight with an exponential-like distribution of lesion sizes. Significant correlations (r = 0.52, 0.58, and 0.63, P < 0.001) were observed across three glasshouse tests of 54 accessions including wild species using the droplet method. The most resistant accessions included wild species: one accession of Solanum arcanum, three accessions of Solanum peruvianum, one accession of Solanum neorickii, and one of Solanum chilense. Solanum pennellii and Solanum pimpinellifolium accessions were susceptible, whereas Solanum habrochaites and Solanum lycopersicum accessions ranged from susceptible to moderately resistant. The droplet test method is simple to apply, offers a fine discrimination of early blight resistance levels, and allows objective evaluation. © 2007 The Phytopathological Society of Japan and Springer-Verlag.
引用
收藏
页码：96 / 103
页数：7
相关论文
共 23 条
  • [1] Banerjee M.K., Chhabra M.L., Saini P.S., Responses of tomato cultivars to Alternaria blight, Test Agrochem Cult, 19, pp. 50-51, (1998)
  • [2] Barksdale T.H., Resistance of tomato seedlings to early blight, Phytopathology, 59, pp. 443-446, (1969)
  • [3] Bonde R., Physiological strains of Alternaria solani, Phytopathology, 19, pp. 533-548, (1929)
  • [4] Foolad M.R., Ntahimpera N., Christ B.J., Lin G.Y., Comparison of field, greenhouse, and detached-leaflet evaluations of tomato germ plasm for early blight resistance, Plant Dis, 84, pp. 967-972, (2000)
  • [5] Gardner R.G., NC EBR-1 and NC EBR-2 early blight resistant tomato breeding lines, HortScience, 23, pp. 779-781, (1988)
  • [6] Gardner R.G., Greenhouse disease screen facilitates breeding resistance to tomato early blight, HortScience, 25, pp. 222-223, (1990)
  • [7] Gardner R.G., Shoemaker P.B., Mountain Supreme early blight-resistant hybrid tomato and its parents, NC EBR-3 and NC EBR-4, HortScience, 34, pp. 745-746, (1999)
  • [8] Henning R.G., Alexander L.J., Evidence of existence of physiologic races of Alternaria solani, Plant Dis Rep, 43, pp. 298-308, (1959)
  • [9] Kalloo G., Banerjee M.K., Early blight resistance in Lycopersicum esculentum Mill. transferred from L. pimpinellifolium (L.) Mill. and L. hirsutum f. glabratum Mull, Gartenbauwissenschaft, 58, pp. 238-239, (1993)
  • [10] Locke S.B., A method for measuring resistance to defoliation diseases in tomato and other Lycopersicon species, Phytopathology, 38, pp. 937-942, (1948)
123