Isolation and characterization of endophytic fungi from Camptotheca acuminata

被引：0
作者：
Xiaowei Ding
Kaihui Liu
Baiwan Deng
Wenqiang Chen
Wenjun Li
Feihu Liu
机构：
[1] Yunnan University,School of Life Sciences
[2] Shaanxi University of Technology,Shaanxi Province Engineering Technology Research Center of Edible and Medicinal Fungi
来源：
World Journal of Microbiology and Biotechnology | 2013年 / 29卷
关键词：
Endophytic fungi; 9-Methoxycamptothecin; Natural compounds;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, a total of 161 endophytic fungal isolates from Camptotheca acuminata were obtained and classified to 16 taxa according to morphological and molecular analysis. These taxa were composed of 2 frequent genera (Botryosphaeria and Fusarium) and 14 infrequent groups such as Xylaria, Diaporthe, Rhizopus, Epicoccum, and Preussia, demonstrating that fungal endophytes in C. acuminata were highly abundant and diverse. Antimicrobial activity screening using filter-paper diffusion method showed that 47.6 % of the tested isolates had antimicrobial activity against at least one of the test microorganisms. Screening of fungal endophyte-derived camptothecin analogues by TLC and LC–MS/MS3 demonstrated that a strain Botryosphaeria dothidea, X-4 could produce 9-methoxycamptothecin (9-MCPT) when cultured in Sabouraud’s dextrose broth for 12 days under shake flask and bench-scale fermention conditions. This work showed that the fungal endophytes from C. acuminata could be an alternative source for the production of 9-MCPT and other natural antimicrobial compounds.
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页码：1831 / 1838
页数：7
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