The comparison of the viability of crushed, morselized and diced cartilage grafts: a confocal microscopic study

被引：0
作者：
Gurkan Kayabasoglu
Elvan Ozbek
Sevinc Yanar
Fikrettin Sahin
Osman Nuri Keles
Mahmut Sinan Yilmaz
Mehmet Guven
机构：
[1] Sakarya University,Department of Otorhinolaryngology, Faculty of Medicine
[2] Sakarya University,Department of Histology and Embryology, Faculty of Medicine
[3] Yeditepe University,Department of Genetics and Bioengineering, Faculty of Engineering and Architecture
[4] Ataturk University,Department of Histology and Embryology, Faculty of Medicine
来源：
European Archives of Oto-Rhino-Laryngology | 2015年 / 272卷
关键词：
Confocal microscopy; Cartilage; Cell viability; Experimental; Rhinoplasty; Morselization; Graft; Diced; Crushing; Nasal surgery;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To compare the cellular viability of diced, crushed, and morselized cartilage used in nasal surgeries. In this study, cartilage was extracted from the ears of seven New Zealand rabbits and was subsequently either diced, crushed or morselized to an amorphous state, or left unmodified. The four types of grafts were then implanted in the back regions of the rabbits. After 3 months, the cellular viability from four groups was compared to a control group using confocal microscopy. Analysis of the data obtained from the enumeration of live cells showed no statistically significant difference between the unmodified graft group and the control group. The diced, crushed, and morselized cartilage groups did show a statistically significant difference in terms of live cell count with the highest number of live cells in diced cartilage group. A statistically significant decrease in live cell count was detected in crushed cartilage group. Our study shows that the viability of cells in diced cartilage grafts is greater than those in crushed or morselized cartilage grafts.
引用
收藏
页码：1135 / 1142
页数：7
相关论文
共 86 条
  • [1] Ansari K(2008)Grafts and implants in rhinoplasty—Techniques and long-term results Op Tech Otolaryngol 19 42-58
  • [2] Asaria J(1993)Crushed Cartilage Grafts over Alar Dome Reduction in Open Rhinoplasty Plast Reconstr Surg 92 352-37
  • [3] Hilger P(2007)The effects of radiofrequency energy probe speed and application force on chondrocyte viability Vet Comp Orthop Traumatol. 20 34-136
  • [4] Adamson PA(1984)The Unbiased estimation of number and size of arbitrary particles using the dissector J Microsc 134 127-571
  • [5] Hamra ST(2001)Quantification of laser-induced cartilage injury by confocal microscopy in an ex vivo model J Bone Joint Surg Am 83 566-263
  • [6] Lu Y(1987)The efficiency of systematic sampling in stereology and its prediction J Microsc 147 229-128
  • [7] Meyer ML(2013)A comparison of lipid storage in J Microbiol Methods 95 122-212
  • [8] Bogdanske JJ(1985) and J Microsc 138 203-193
  • [9] Markel MD(2008) using laser scanning confocal microscopy Arch Facial Plast Surg 10 187-351
  • [10] Sterio DC(1993)Unbiased estimation of particle density in the tandem scanning reflected light microscope Plast Reconstr Surg 92 346-287
123456789