HPV DNA is associated with a subset of Schneiderian papillomas but does not correlate with p16INK4a immunoreactivity

被引：19

作者：

Shah A.A. ^{[1
]}

Evans M.F. ^{[1
]}

Adamson C.S.-C. ^{[1
]}

Peng Z. ^{[1
]}

Rajendran V. ^{[1
]}

Cooper K. ^{[1
,2
]}

机构：

[1] Department of Pathology, University of Vermont, Burlington, VT

[2] Department of Pathology, Fletcher Allen Health Care, Burlington, VT

来源：

Head and Neck Pathology | 2010年 / 4卷 / 2期

关键词：

Human papillomavirus; p16[!sup]INK4a[!/sup; Schneiderian papilloma; Sinonasal papilloma;

D O I：

10.1007/s12105-010-0176-4

中图分类号：

学科分类号：

摘要：

This study investigated the role of human papillomavirus (HPV) in Schneiderian papillomas (SPs) to determine whether HPV is associated with the pathogenesis of particular histologic subtypes and whether p16INK4a can be used as a surrogate marker for HPV detection. Twenty-seven papilloma specimens (19 inverted [IPs], 6 exophytic [EPs], 1 oncocytic [OP] and 1 mixed) were collected from 23 patients. Purified SP DNA extracts were tested for HPV by PCR using GP5 +/GP6 + primers; HPV genotyping was performed by dot blot hybridization. PCR positive specimens were screened for HPV by biotinyl-tyramide-based chromogenic in situ hybridization (CISH). Immunohistochemsistry (IHC) for the HPV L1 capsid protein and for p16INK4a was performed on all specimens. HPV was detected by PCR in 16/27 (59.3%) SPs; 9/19 (47.4%) IPs; 6/6 (100%) EPs [p = 0.051], and 1/1 (100%) mixed SP. HPV was not detected in the single OP. High risk genotypes were detected in 4/9 IPs (44.4%) and 0/6 EPs (0%) [p = 0.10]. Seven of 16 PCR positive SPs were also CISH positive for HPV: 5/6 EPs (83.3%) and 1/9 IP (11.1%) [p = 0.01]. IHC for the L1 capsid protein was positive in 2 SPs (1 EP and 1 mixed). p16INK4a staining was seen in 14/16 (87.5%) PCR positive SPs and in 10/11 (90.9%) PCR negative SPs (p = 1.00). In summary, this study demonstrates a strong association between HPV and EPs, however, its role in IPs remains less well-defined. Further, p16INK4a is not a useful surrogate marker for HPV detection across the various SPs. © 2010 Humana.

引用

页码：106 / 112

页数：6

共 31 条

[1] Perez-Ordonez B., Special tumours of the head and neck, Curr Diagn Pathol, 9, pp. 366-383, (2003)
[2] Pathology and Genetics of Head and Neck Tumours. World Health Organization Classification of Tumours, (2005)
[3] Hyams V.J., Papillomas of the nasal cavity and paranasal sinuses. A clinicopathological study of 315 cases, Ann Otol Rhinol Laryngol, 80, pp. 192-206, (1971)
[4] Syrjanen S., Happonen R.P., Virolainen E., Et al., Detection of human papillomavirus (HPV) structural antigens and DNA types in inverted papillomas and squamous cell carcinomas of the nasal cavities and paranasal sinuses, Acta Otolaryngol, 104, pp. 334-341, (1987)
[5] Syrjanen K.J., HPV infections in benign and malignant sinonasal lesions, J Clin Pathol, 56, pp. 174-181, (2003)
[6] Gaffey M.J., Frierson H.F., Weiss L.M., Et al., Human papillomavirus and Epstein-Barr virus in sinonasal Schneiderian papillomas. An in situ hybridization and polymerase chain reaction study, Am J Clin Pathol, 106, pp. 475-482, (1996)
[7] Judd R., Zaki S.R., Coffield L.M., Et al., Sinonasal papillomas and HPV: HPV 11 detected in fungiform Schneiderian papillomas by in situ hybridization and PCR, Hum Pathol, 22, pp. 550-556, (1991)
[8] Weiner J.S., Sherris D., Kasperbauer J., Et al., Relationship of HPV to Schneiderian papillomas, Laryngoscope, 109, pp. 21-26, (1999)
[9] Buchwald C., Franzmann M.B., Jacobsen G.K., Et al., Human papillomavirus (HPV) in sinonasal papillomas: A study of 78 cases using in situ hybridization and polymerase chain reaction, Laryngoscope, 105, pp. 66-71, (1995)
[10] Lawson W., Schlecht N.F., Brandwein-Gensler M., The role of the human papillomavirus in the pathogenesis of Schneiderian inverted papillomas: An analytic overview of the evidence, Head Neck Pathol, 2, pp. 49-59, (2008)

← 1 2 3 4 →