Triacylglycerols sequester monotopic membrane proteins to lipid droplets

被引：0
作者：
Lucie Caillon
Vincent Nieto
Pauline Gehan
Mohyeddine Omrane
Nicolas Rodriguez
Luca Monticelli
Abdou Rachid Thiam
机构：
[1] Laboratoire de Physique de l’École Normale Supérieure,
[2] ENS,undefined
[3] Université PSL,undefined
[4] CNRS,undefined
[5] Sorbonne Université,undefined
[6] Université de Paris,undefined
[7] University of Lyon,undefined
[8] CNRS,undefined
[9] Molecular Microbiology and Structural Biochemistry (MMSB,undefined
[10] UMR 5086),undefined
[11] Sorbonne Universités,undefined
[12] UPMC Univ Paris 06,undefined
[13] Ecole Normale Supérieure,undefined
[14] CNRS,undefined
[15] Laboratoire des Biomolécules (LBM),undefined
[16] 4 place Jussieu,undefined
[17] Département de Chimie,undefined
[18] Ecole Normale Supérieure,undefined
[19] PSL Research University,undefined
[20] UPMC Univ Paris 06,undefined
[21] CNRS,undefined
[22] Laboratoire des Biomolécules (LBM),undefined
来源：
Nature Communications | / 11卷
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摘要：
Triacylglycerols (TG) are synthesized at the endoplasmic reticulum (ER) bilayer and packaged into organelles called lipid droplets (LDs). LDs are covered by a single phospholipid monolayer contiguous with the ER bilayer. This connection is used by several monotopic integral membrane proteins, with hydrophobic membrane association domains (HDs), to diffuse between the organelles. However, how proteins partition between ER and LDs is not understood. Here, we employed synthetic model systems and found that HD-containing proteins strongly prefer monolayers and returning to the bilayer is unfavorable. This preference for monolayers is due to a higher affinity of HDs for TG over membrane phospholipids. Protein distribution is regulated by PC/PE ratio via alterations in monolayer packing and HD-TG interaction. Thus, HD-containing proteins appear to non-specifically accumulate to the LD surface. In cells, protein editing mechanisms at the ER membrane would be necessary to prevent unspecific relocation of HD-containing proteins to LDs.
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