A method for detergent-free isolation of membrane proteins in their local lipid environment

被引：0

作者：

Sarah C Lee

Tim J Knowles

Vincent L G Postis

Mohammed Jamshad

Rosemary A Parslow

Yu-pin Lin

Adrian Goldman

Pooja Sridhar

Michael Overduin

Stephen P Muench

Timothy R Dafforn

机构：

[1] School of Biosciences,Department of Biosciences, Division of Biochemistry

[2] University of Birmingham,Department of Biochemistry

[3] School of Cancer Sciences,undefined

[4] University of Birmingham,undefined

[5] School of Biomedical Sciences,undefined

[6] Faculty of Biological Sciences,undefined

[7] University of Leeds,undefined

[8] Biomedicine Research Group,undefined

[9] Faculty of Health and Social Sciences,undefined

[10] Leeds Beckett University,undefined

[11] University of Helsinki,undefined

[12] Faculty of Medicine & Dentistry,undefined

[13] University of Alberta,undefined

[14] Present address: Department of Biosciences,undefined

[15] University of Birmingham,undefined

[16] Birmingham,undefined

[17] UK.,undefined

来源：

Nature Protocols | 2016年 / 11卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This protocol from Lee et al. describes a method for the extraction of membrane proteins in their native lipid environment using styrene maleic anhydride (SMA) co-polymer. The method is applicable to both prokaryotic and eukaryotic expression systems.

引用

页码：1149 / 1162

页数：13

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