A method for detergent-free isolation of membrane proteins in their local lipid environment

被引：0
|
作者：
Sarah C Lee
Tim J Knowles
Vincent L G Postis
Mohammed Jamshad
Rosemary A Parslow
Yu-pin Lin
Adrian Goldman
Pooja Sridhar
Michael Overduin
Stephen P Muench
Timothy R Dafforn
机构：
[1] School of Biosciences,Department of Biosciences, Division of Biochemistry
[2] University of Birmingham,Department of Biochemistry
[3] School of Cancer Sciences,undefined
[4] University of Birmingham,undefined
[5] School of Biomedical Sciences,undefined
[6] Faculty of Biological Sciences,undefined
[7] University of Leeds,undefined
[8] Biomedicine Research Group,undefined
[9] Faculty of Health and Social Sciences,undefined
[10] Leeds Beckett University,undefined
[11] University of Helsinki,undefined
[12] Faculty of Medicine & Dentistry,undefined
[13] University of Alberta,undefined
[14] Present address: Department of Biosciences,undefined
[15] University of Birmingham,undefined
[16] Birmingham,undefined
[17] UK.,undefined
来源：
Nature Protocols | 2016年 / 11卷
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学科分类号：
摘要：
This protocol from Lee et al. describes a method for the extraction of membrane proteins in their native lipid environment using styrene maleic anhydride (SMA) co-polymer. The method is applicable to both prokaryotic and eukaryotic expression systems.
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页码：1149 / 1162
页数：13
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