Targeted site-specific cleavage of HIV-1 viral Rev responsive element by copper aminoglycosides

被引：0

作者：

Alavattam Sreedhara

J.A. Cowan

机构：

[1] Evans Laboratory of Chemistry,

[2] The Ohio State University,undefined

[3] 100 West 18th Avenue,undefined

[4] Columbus,undefined

[5] OH 43210,undefined

[6] USA,undefined

[7] Present address: Molecular,undefined

[8] Cellular and Developmental Biology,undefined

[9] Yale University,undefined

[10] 219 Prospect Street,undefined

[11] New Haven,undefined

[12] CT 06520,undefined

[13] USA,undefined

来源：

JBIC Journal of Biological Inorganic Chemistry | 2001年 / 6卷

关键词：

HIV Rev response element RNA Cleavage Aminoglycoside Drug target Copper;

D O I：

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中图分类号：

学科分类号：

摘要：

Site-specific cleavage of the HIV-1 viral Rev responsive element by copper aminoglycosides is reported under physiological conditions. This bubble and stem-loop RNA structure is efficiently targeted at micromolar concentrations of complex. The specificity of cleavage of structured viral RNA relative to a non-cognate tRNAPhe of well-defined secondary and tertiary structure is demonstrated. Cleavage products from simpler substrates [diribonucleotide (ApA) and 2′,3′-cyclic monophosphate ester (cAMP)] were analyzed by 31P NMR and demonstrate a hydrolytic mechanism in the absence of external redox agents. These results demonstrate copper aminoglycosides to be highly efficient chemical nucleases with a targeting capability for viral RNA and suggest a novel methodology to counter RNA viruses.

引用

页码：166 / 172

页数：6