PCR Screening of Enterococcus faecalis from Mixture of Lactic Acid Bacteria Using Bile Salt Hydrolase Gene as a Selective Marker

被引:0
|
作者
Hye Min Yoon
Su Jin Song
Ji Su Ko
Hye Kyoung Yoon
Su Jeong Kim
So Jin Kim
Ji Woon Kim
Min Yoo
机构
[1] Keimyung University,Department of Biological Sciences
来源
Indian Journal of Microbiology | 2020年 / 60卷
关键词
Food quarantine; PCR primer; Bile salt hydrolase gene;
D O I
暂无
中图分类号
学科分类号
摘要
It is very important to rapidly detect the contamination of Enterococcus faecalis in fermented foods such as Korean Kimchi to maintain its freshness since Kimchi is exported to all over the world. However, gene sequence of E. faecalis is very similar among various Lactobacillus. So, there have been difficulties in its screening. We have designed primers based on Bile salt hydrolase gene of E. faecalis and applied them to PCR test. PCR band was identified only from E. faecalis and only from the mixture contaminated with E. faecalis. It means that the primers we designed are highly specific for distinguishing contamination of E. faecalis. It will be possible to precisely screen within 1 h, which will greatly contribute to the prevention of food poisoning and quick quarantine.
引用
收藏
页码:251 / 253
页数:2
相关论文
共 44 条
  • [1] PCR Screening of Enterococcus faecalis from Mixture of Lactic Acid Bacteria Using Bile Salt Hydrolase Gene as a Selective Marker
    Yoon, Hye Min
    Song, Su Jin
    Ko, Ji Su
    Yoon, Hye Kyoung
    Kim, Su Jeong
    Kim, So Jin
    Kim, Ji Woon
    Yoo, Min
    INDIAN JOURNAL OF MICROBIOLOGY, 2020, 60 (02) : 251 - 253
  • [2] Screening of lactic acid bacteria for bile salt hydrolase activity
    Tanaka, H
    Doesburg, K
    Iwasaki, T
    Mierau, I
    JOURNAL OF DAIRY SCIENCE, 1999, 82 (12) : 2530 - 2535
  • [4] Analysis of acid and bile salt tolerance and study on bile salt hydrolase in lactic acid bacteria
    Chen, S., 1600, Chinese Institute of Food Science and Technology (13):
  • [5] A novel vector for lactic acid bacteria that uses a bile salt hydrolase gene as a potential food-grade selection marker
    Yin, Sheng
    Zhai, Zhengyuan
    Wang, Guohong
    An, Haoran
    Luo, Yunbo
    Hao, Yanling
    JOURNAL OF BIOTECHNOLOGY, 2011, 152 (1-2) : 49 - 53
  • [6] Inhibition of Listeria monocytogenes by lactic acid bacteria with bile salt hydrolase activity
    Taranto, MP
    de Llano, DG
    de Valdez, GF
    MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL, 2000, 55 (01): : 22 - 24
  • [7] A highly active bile salt hydrolase from Enterococcus faecalis shows positive cooperative kinetics
    Chand, Deepak
    Ramasamy, Sureshkumar
    Suresh, C. G.
    PROCESS BIOCHEMISTRY, 2016, 51 (02) : 263 - 269
  • [8] Bile salt hydrolase activity is present in nonintestinal lactic acid bacteria at an intermediate level
    Ru, Xiao
    Zhang, Chuang-Chuang
    Yuan, Ya-Hong
    Yue, Tian-Li
    Guo, Chun-Feng
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 103 (02) : 893 - 902
  • [9] Bile salt hydrolase activity is present in nonintestinal lactic acid bacteria at an intermediate level
    Xiao Ru
    Chuang-Chuang Zhang
    Ya-Hong Yuan
    Tian-Li Yue
    Chun-Feng Guo
    Applied Microbiology and Biotechnology, 2019, 103 : 893 - 902
  • [10] Screening of bile salt hydrolase-producing lactic acid bacteria and evaluation of cholesterol-lowering activity in vitro
    Lv, Chenglong
    Pang, Xinyi
    Sun, Jing
    Li, Xiangfei
    Lu, Yingjian
    FOOD BIOSCIENCE, 2024, 62