Indirect Organogenesis from the Leaf Explants of Medicinally Important Plant Curculigo orchioides Gaertn

被引：0
|
作者：
S. Dhenuka
P. Balakrishna
Ajith Anand
机构：
[1] M S Swami nathan Research Foundation,
来源：
Journal of Plant Biochemistry and Biotechnology | 1999年 / 8卷
关键词：
indirect organogenesis; propagation; medicinal plant;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Curculigo orchioides Gaertn is an important medicinal plant of commercial value. A protocol for indirect regeneration from the leaf explants was developed. The leaf explants on MS (Murashige & Skoog) basal medium fortified with 6-benzylaminopurine (9μM) gave rise to proliferating green callus. The calli regenerated shoots on subculturing in the MS medium with 1.1μM 6-benzylaminopurine which was the optimum concentration for multiplication. Rooting was observed in MS medium supplemented with α-napthaleneacetic acid (5.3μM), indole-3-butyric acid (1.2μM), and on MS basal medium free of plant growth regulators. The rooted plants were hardened and transferred to field with 80% survival.
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页码：113 / 115
页数：2
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