Expression of Somatostatin Receptor mRNA in Human Meningiomas and their Implication in in vitro Antiproliferative Activity

被引：3
作者：
Sara Arena
Federica Barbieri
Stefano Thellung
Paolo Pirani
Alessandro Corsaro
Valentina Villa
Patrizia Dadati
Alessandra Dorcaratto
Gabriella Lapertosa
Jean-Louis Ravetti
Renato Spaziante
Gennaro Schettini
Tullio Florio
机构：
[1] University of Genova,Section of Pharmacology, Department of Oncology, Biology and Genetics
[2] University of Genova,Section of Neurosurgery DISCAT
[3] University of Genova,Section of Pathology, DICMI
[4] University of Genova,Division of Neurosurgery, Department of Neuroscience, Opthalmology and Genetics
[5] Pharmacology and Neurosciences,Section of Pathology
[6] National Institute for Cancer Research (IST),undefined
[7] San Martino Hospital,undefined
来源：
Journal of Neuro-Oncology | 2004年 / 66卷
关键词：
meningioma; primary cultures; proliferation; RT–PCR; somatostatin receptors;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Somatostatin receptors (SSTRs) have been detected in many normal and malignant tissues. This wide expression has been used for diagnostic, prognostic and therapeutic purposes. Five SSTR subtypes (SSTR 1–5) have been identified whose activation is responsible for the signal transduction through many different intracellular pathways. In the present study the expression of SSTR mRNA was determined by reverse-transcriptase (RT)–PCR in 42 meningiomas. About 88% of the tumors analyzed (37/42) were positive for at least one of the five SSTR subtypes displaying a variable pattern of expression of the different SSTR subtypes. SSTR1 and SSTR2 were the most frequently mRNA detected (69% and 79% of the sample analyzed, respectively). The other subtypes were found in the 43%, 33% and 33% of cases for SSTR3, SSTR4 and SSTR5, respectively. In 22, out of 42 patients (52%) three or more SSTRs were detected. The expression of the different SSTR subtypes did not correlate with the expression of bcl-2 (apoptosis-associated protein) and MIB-1 (a proliferation marker), assessed by immunohistochemistry in a series of 34 tumor samples, while a correlation between the expression of SSTR3 and p53 was observed (p = 0.08). To evaluate a possible role of SSTR in the control of human meningioma cell proliferation, seven primary cell cultures obtained from fresh meningioma surgical tissues, were analyzed for their proliferative behavior by MTT assay and for their response to SST by [3H]-thymidine incorporation. In four out of six tumors (in one case no SSTR were detected) the treatment with SST caused a significant inhibition of DNA synthesis induced by the tumor-promoter phorbol myristate acetate. The evidence of the expression of SSTRs, mainly of SSTR2, in this series of specimens we analyzed altogether with in vitro antiproliferative effects of SST may open interesting perspectives for the diagnosis and the therapy of meningiomas.
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页码：155 / 166
页数：11
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